Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar

The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months withou...

Descripción completa

Detalles Bibliográficos
Autores principales: Wuthiekanun, Vanaporn, Amornchai, Premjit, Langla, Sayan, Oyuchua, Malinee, Day, Nicholas P. J., Limmathurotsakul, Direk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2014
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313312/
https://www.ncbi.nlm.nih.gov/pubmed/25253789
http://dx.doi.org/10.1128/JCM.02273-14
Descripción
Sumario:The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye.

Ejemplares similares