Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar

The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months withou...

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Autores principales: Wuthiekanun, Vanaporn, Amornchai, Premjit, Langla, Sayan, Oyuchua, Malinee, Day, Nicholas P. J., Limmathurotsakul, Direk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2014
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313312/
https://www.ncbi.nlm.nih.gov/pubmed/25253789
http://dx.doi.org/10.1128/JCM.02273-14
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author Wuthiekanun, Vanaporn
Amornchai, Premjit
Langla, Sayan
Oyuchua, Malinee
Day, Nicholas P. J.
Limmathurotsakul, Direk
author_facet Wuthiekanun, Vanaporn
Amornchai, Premjit
Langla, Sayan
Oyuchua, Malinee
Day, Nicholas P. J.
Limmathurotsakul, Direk
author_sort Wuthiekanun, Vanaporn
collection PubMed
description The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye.
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spelling pubmed-43133122015-02-02 Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar Wuthiekanun, Vanaporn Amornchai, Premjit Langla, Sayan Oyuchua, Malinee Day, Nicholas P. J. Limmathurotsakul, Direk J Clin Microbiol Bacteriology The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye. American Society for Microbiology 2014-12 /pmc/articles/PMC4313312/ /pubmed/25253789 http://dx.doi.org/10.1128/JCM.02273-14 Text en Copyright © 2014 Wuthiekanun et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Bacteriology
Wuthiekanun, Vanaporn
Amornchai, Premjit
Langla, Sayan
Oyuchua, Malinee
Day, Nicholas P. J.
Limmathurotsakul, Direk
Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar
title Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar
title_full Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar
title_fullStr Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar
title_full_unstemmed Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar
title_short Maintenance of Leptospira Species in Leptospira Vanaporn Wuthiekanun Agar
title_sort maintenance of leptospira species in leptospira vanaporn wuthiekanun agar
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313312/
https://www.ncbi.nlm.nih.gov/pubmed/25253789
http://dx.doi.org/10.1128/JCM.02273-14
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