Cargando…

Evaluation of in-house polymerase chain reaction assay sensitivity, can it be utilized in limited-resources settings?

Background: Polymerase chain reaction (PCR) assay has widely used for the detection of tuberculosis (TB). This study tried to compare in-house PCR with some well-known commercial PCR kits for detection of TB agent. Methods: Clinical samples obtained from 620 TB suspected patients were analyzed for t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Dorudinia, Atosa, Shamaei, Masoud, Karimi, Shirin, Javadi, Alireza, Mohammadi Ziazi, Leila, Pourabdollah, Mihan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Iran University of Medical Sciences 2014
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313456/ https://www.ncbi.nlm.nih.gov/pubmed/25679005

Ejemplares similares

Qualification Study of Two Genomic DNA Extraction Methods in Different Clinical Samples
por: Javadi, Alireza, et al.
Publicado: (2014)

Clonality of the Immunoglobulin Heavy Chain Genes in B Cell Non-Hodgkin Lymphoma Using Semi-Nested PCR
por: Taheri, Zohreh Mohammad, et al.
Publicado: (2011)

DNA sequencing validation by PCR-RFLP for evaluating butyrophilin-like 2 rs2076530 polymorphism in Iranian patients with sarcoidosis
por: Javadi, Alireza, et al.
Publicado: (2021)

Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis
por: Karimi, Shirin, et al.
Publicado: (2014)

The presence of mycobacterial antigens in sarcoidosis associated granulomas
por: Shamaei, Masoud, et al.
Publicado: (2017)

Diffuse alveolar damage and thrombotic microangiopathy are the main histopathological findings in lung tissue biopsy samples of COVID-19 patients
por: Sadegh Beigee, Farahnaz, et al.
Publicado: (2020)

The Frequency of Epidermal Growth Factor Receptor (EGFR) Mutation in Patients with Lung Adenocarcinoma Referred to a Lung Diseases Hospital; A Cross-Sectional Study from Iran
por: Mohammadi, Sotoudeh, et al.
Publicado: (2022)

The Relation between Polymorphisms in Exon 5 and Exon 6 of GSTP1 Gene and the Risk of Lung Cancer in Iranian People
por: Shahsavari, Gholamreza, et al.
Publicado: (2019)

Differential role of gpaB and sidA gene expressions in relation to virulence in Aspergillus species from patients with invasive aspergillosis
por: Ghods, Nayereh, et al.
Publicado: (2018)

Microbial contamination of single- and multiple-dose vials after opening in a pulmonary teaching hospital
por: Baniasadi, Shadi, et al.
Publicado: (2013)

Asymptomatic Giant Lipoma of the Right Ventricular Outflow Tract: A Case Report
por: Bayat, Fariba, et al.
Publicado: (2017)

Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction
por: McElroy, Sheri M., et al.
Publicado: (2005)

Extracellular Vesicles from Serum of Mycobacteria Patients Accelerate Expression of Apoptosis miRNAs and Facilitate THP-1 Monocyte Cell Death
por: Javadi, Alireza, et al.
Publicado: (2022)

Insertion/Deletion Polymorphisms and Serum Angiotensin-converting Enzyme Levels in Iranian Patients with Sarcoidosis
por: JAVADI, Alireza, et al.
Publicado: (2016)

Diagnostic accuracy of two multiplex real-time polymerase chain reaction assays for the diagnosis of meningitis in children in a resource-limited setting
por: Khumalo, Jermaine, et al.
Publicado: (2017)

Extracellular vesicles from serum samples of mycobacteria patients induced cell death of THP-1 monocyte and PBMC
por: Javadi, Alireza, et al.
Publicado: (2022)

A Twenty-Year-Old Woman with Hemoptysis
por: Darazam, Ilad Alavi, et al.
Publicado: (2011)

Visceral Leishmaniasis in Two Brothers; Diagnostic Dilemma Due to Hemophagocytic Syndrome
por: Khalilzadeh, Soheila, et al.
Publicado: (2013)

Acremonium Pneumonia: Case Report and Literature Review
por: Fakharian, Atefeh, et al.
Publicado: (2015)

Synovial Sarcoma of the Esophagus: A Case Report and Review of Literature
por: Doroudinia, Abtin, et al.
Publicado: (2017)

Evaluation of cardiomyopathy diagnosis in heart transplant recipients: comparison of echocardiographic and pathologic classification
por: Behzadnia, Neda, et al.
Publicado: (2021)

Comparison of Parasite Burden Using Real-Time Polymerase Chain Reaction Assay and Limiting Dilution Assay in Leishmania major Infected Mouse
por: GHOTLOO, Somayeh, et al.
Publicado: (2015)

A 60 Year-Old Man with AIDS and Pneumonia
por: Tabarsi, Payam, et al.
Publicado: (2013)

Flock-dependent exploitation of a limited resource in House Sparrow
por: Ligorio, Elisa, et al.
Publicado: (2020)

Delayed post‐pneumonectomy empyema necessitans caused by Aspergillus flavus : An unusual report
por: Hakamifard, Atousa, et al.
Publicado: (2022)

Polymerase chain reaction–based assays for the diagnosis of human brucellosis
por: Wang, Ying, et al.
Publicado: (2014)

Validating Real-Time Polymerase Chain Reaction (PCR) Assays
por: Smith, Melvyn
Publicado: (2021)

Myocarditis following rAd26 and rAd5 vector‐based COVID‐19 vaccine: case report
por: Naghashzadeh, Farah, et al.
Publicado: (2022)

Hypermetabolic Thyroid Incidentaloma on Positron Emission Tomography: Review of Laboratory, Radiologic, and Pathologic Characteristics
por: Bakhshayesh Karam, Mehrdad, et al.
Publicado: (2017)

Photobiomodulation Improves Serum Cytokine Response in Mild to Moderate COVID-19: The First Randomized, Double-Blind, Placebo Controlled, Pilot Study
por: Marashian, Seyed Mehran, et al.
Publicado: (2022)

Real-time digital polymerase chain reaction (PCR) as a novel technology improves limit of detection for rare allele assays
por: Xu, Jiachen, et al.
Publicado: (2021)

Real-Time Polymerase Chain Reaction Assay for the Diagnosis of Pulmonary Tuberculosis
por: Wiwanitkit, Viroj
Publicado: (2015)

Necrotizing Sarcoid Granulomatosis with Pneumothorax
por: Pourabdollah, Mihan, et al.
Publicado: (2019)

The impact of COVID-19 on TB in Iran: An illustrative study
por: Tabarsi, Payam, et al.
Publicado: (2023)

Effects of Exogenous Melatonin on MAM Induced Lung Injury and Lung Development in Mice Offspring
por: Azizi, Maryam, et al.
Publicado: (2020)

Polymerase chain reaction test for diagnosis of infectious uveitis
por: Fekri, Sahba, et al.
Publicado: (2023)

The Frequency of Human Papillomavirus Positivity in Iranian Patients with Head and Neck Squamous Cell Carcinoma
por: Rezaei, Mitra, et al.
Publicado: (2021)

A 26 Year-Old Woman with AIDS and Pneumonia
por: Yousefzadeh, Amir, et al.
Publicado: (2013)

A new ataxia-telangiectasia mutation in an 11-year-old female
por: Mortaz, Esmaeil, et al.
Publicado: (2017)

In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity
por: Kalita, Manash Jyoti, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_jbsma9tbiq267jm40thb1i7d9p