Cargando…
Gene Therapy for Bone Engineering
Bone has an intrinsic healing capacity that may be exceeded when the fracture gap is too big or unstable. In that moment, osteogenic measures need to be taken by physicians. It is important to combine cells, scaffolds and growth factors, and the correct mechanical conditions. Growth factors are clin...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313589/ https://www.ncbi.nlm.nih.gov/pubmed/25699253 http://dx.doi.org/10.3389/fbioe.2015.00009 |
_version_ | 1782355239684276224 |
---|---|
author | Balmayor, Elizabeth Rosado van Griensven, Martijn |
author_facet | Balmayor, Elizabeth Rosado van Griensven, Martijn |
author_sort | Balmayor, Elizabeth Rosado |
collection | PubMed |
description | Bone has an intrinsic healing capacity that may be exceeded when the fracture gap is too big or unstable. In that moment, osteogenic measures need to be taken by physicians. It is important to combine cells, scaffolds and growth factors, and the correct mechanical conditions. Growth factors are clinically administered as recombinant proteins. They are, however, expensive and needed in high supraphysiological doses. Moreover, their half-life is short when administered to the fracture. Therefore, gene therapy may be an alternative. Cells can constantly produce the protein of interest in the correct folding, with the physiological glycosylation and in the needed amounts. Genes can be delivered in vivo or ex vivo by viral or non-viral methods. Adenovirus is mostly used. For the non-viral methods, hydrogels and recently sonoporation seem to be promising means. This review will give an overview of recent advancements in gene therapy approaches for bone regeneration strategies. |
format | Online Article Text |
id | pubmed-4313589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-43135892015-02-19 Gene Therapy for Bone Engineering Balmayor, Elizabeth Rosado van Griensven, Martijn Front Bioeng Biotechnol Bioengineering and Biotechnology Bone has an intrinsic healing capacity that may be exceeded when the fracture gap is too big or unstable. In that moment, osteogenic measures need to be taken by physicians. It is important to combine cells, scaffolds and growth factors, and the correct mechanical conditions. Growth factors are clinically administered as recombinant proteins. They are, however, expensive and needed in high supraphysiological doses. Moreover, their half-life is short when administered to the fracture. Therefore, gene therapy may be an alternative. Cells can constantly produce the protein of interest in the correct folding, with the physiological glycosylation and in the needed amounts. Genes can be delivered in vivo or ex vivo by viral or non-viral methods. Adenovirus is mostly used. For the non-viral methods, hydrogels and recently sonoporation seem to be promising means. This review will give an overview of recent advancements in gene therapy approaches for bone regeneration strategies. Frontiers Media S.A. 2015-02-02 /pmc/articles/PMC4313589/ /pubmed/25699253 http://dx.doi.org/10.3389/fbioe.2015.00009 Text en Copyright © 2015 Balmayor and van Griensven. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Balmayor, Elizabeth Rosado van Griensven, Martijn Gene Therapy for Bone Engineering |
title | Gene Therapy for Bone Engineering |
title_full | Gene Therapy for Bone Engineering |
title_fullStr | Gene Therapy for Bone Engineering |
title_full_unstemmed | Gene Therapy for Bone Engineering |
title_short | Gene Therapy for Bone Engineering |
title_sort | gene therapy for bone engineering |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313589/ https://www.ncbi.nlm.nih.gov/pubmed/25699253 http://dx.doi.org/10.3389/fbioe.2015.00009 |
work_keys_str_mv | AT balmayorelizabethrosado genetherapyforboneengineering AT vangriensvenmartijn genetherapyforboneengineering |