Proteomics-Driven Analysis of Ovine Whey Colostrum

The aim of this study was to shed light in to the complexity of the ovine colostrum proteome, with a specific focus on the low abundance proteins. The ovine colostrum is characterized by a few dominating proteins, as the immunoglobulins, but it also contains less represented protein species, equally...

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Detalles Bibliográficos
Autores principales: Scumaci, Domenica, Trimboli, Francesca, Dell’Aquila, Ludovica, Concolino, Antonio, Pappaianni, Giusi, Tammè, Laura, Vignola, Giorgio, Luciani, Alessia, Morelli, Daniela, Cuda, Giovanni, Boari, Andrea, Britti, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313942/
https://www.ncbi.nlm.nih.gov/pubmed/25643159
http://dx.doi.org/10.1371/journal.pone.0117433
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author Scumaci, Domenica
Trimboli, Francesca
Dell’Aquila, Ludovica
Concolino, Antonio
Pappaianni, Giusi
Tammè, Laura
Vignola, Giorgio
Luciani, Alessia
Morelli, Daniela
Cuda, Giovanni
Boari, Andrea
Britti, Domenico
author_facet Scumaci, Domenica
Trimboli, Francesca
Dell’Aquila, Ludovica
Concolino, Antonio
Pappaianni, Giusi
Tammè, Laura
Vignola, Giorgio
Luciani, Alessia
Morelli, Daniela
Cuda, Giovanni
Boari, Andrea
Britti, Domenico
author_sort Scumaci, Domenica
collection PubMed
description The aim of this study was to shed light in to the complexity of the ovine colostrum proteome, with a specific focus on the low abundance proteins. The ovine colostrum is characterized by a few dominating proteins, as the immunoglobulins, but it also contains less represented protein species, equally important for the correct development of neonates. Ovine colostrum, collected immediately after lambing, was separated by 1D SDS-PAGE. Proteins bands were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. On the basis of the Swiss-Prot database, a total of 343 unique proteins were identified. To our knowledge, this study represents the most comprehensive analysis of ovine colostrum proteome.
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spelling pubmed-43139422015-02-13 Proteomics-Driven Analysis of Ovine Whey Colostrum Scumaci, Domenica Trimboli, Francesca Dell’Aquila, Ludovica Concolino, Antonio Pappaianni, Giusi Tammè, Laura Vignola, Giorgio Luciani, Alessia Morelli, Daniela Cuda, Giovanni Boari, Andrea Britti, Domenico PLoS One Research Article The aim of this study was to shed light in to the complexity of the ovine colostrum proteome, with a specific focus on the low abundance proteins. The ovine colostrum is characterized by a few dominating proteins, as the immunoglobulins, but it also contains less represented protein species, equally important for the correct development of neonates. Ovine colostrum, collected immediately after lambing, was separated by 1D SDS-PAGE. Proteins bands were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. On the basis of the Swiss-Prot database, a total of 343 unique proteins were identified. To our knowledge, this study represents the most comprehensive analysis of ovine colostrum proteome. Public Library of Science 2015-02-02 /pmc/articles/PMC4313942/ /pubmed/25643159 http://dx.doi.org/10.1371/journal.pone.0117433 Text en © 2015 Scumaci et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Scumaci, Domenica
Trimboli, Francesca
Dell’Aquila, Ludovica
Concolino, Antonio
Pappaianni, Giusi
Tammè, Laura
Vignola, Giorgio
Luciani, Alessia
Morelli, Daniela
Cuda, Giovanni
Boari, Andrea
Britti, Domenico
Proteomics-Driven Analysis of Ovine Whey Colostrum
title Proteomics-Driven Analysis of Ovine Whey Colostrum
title_full Proteomics-Driven Analysis of Ovine Whey Colostrum
title_fullStr Proteomics-Driven Analysis of Ovine Whey Colostrum
title_full_unstemmed Proteomics-Driven Analysis of Ovine Whey Colostrum
title_short Proteomics-Driven Analysis of Ovine Whey Colostrum
title_sort proteomics-driven analysis of ovine whey colostrum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313942/
https://www.ncbi.nlm.nih.gov/pubmed/25643159
http://dx.doi.org/10.1371/journal.pone.0117433
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