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Evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice
OBJECTIVE: Introduction of a high-fat diet to mice results in a period of voracious feeding, known as hyperphagia, before homeostatic mechanisms prevail to restore energy intake to an isocaloric level. Acute high-fat diet hyperphagia induces astrocyte activation in the rodent hypothalamus, suggestin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314532/ https://www.ncbi.nlm.nih.gov/pubmed/25685690 http://dx.doi.org/10.1016/j.molmet.2014.10.001 |
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author | Buckman, Laura B. Thompson, Misty M. Lippert, Rachel N. Blackwell, Timothy S. Yull, Fiona E. Ellacott, Kate L.J. |
author_facet | Buckman, Laura B. Thompson, Misty M. Lippert, Rachel N. Blackwell, Timothy S. Yull, Fiona E. Ellacott, Kate L.J. |
author_sort | Buckman, Laura B. |
collection | PubMed |
description | OBJECTIVE: Introduction of a high-fat diet to mice results in a period of voracious feeding, known as hyperphagia, before homeostatic mechanisms prevail to restore energy intake to an isocaloric level. Acute high-fat diet hyperphagia induces astrocyte activation in the rodent hypothalamus, suggesting a potential role of these cells in the homeostatic response to the diet. The objective of this study was to determine physiologic role of astrocytes in the acute homeostatic response to high-fat feeding. METHODS: We bred a transgenic mouse model with doxycycline-inducible inhibition of NFkappaB (NFκB) signaling in astrocytes to determine the effect of loss of NFκB-mediated astrocyte activation on acute high-fat hyperphagia. ELISA was used to measure the levels of markers of astrocyte activation, glial-fibrillary acidic protein (GFAP) and S100B, in the medial basal hypothalamus. RESULTS: Inhibition of NFκB signaling in astrocytes prevented acute high-fat diet-induced astrocyte activation and resulted in a 15% increase in caloric intake (P < 0.01) in the first 24 h after introduction of the diet. CONCLUSIONS: These data reveal a novel homeostatic role for astrocytes in the acute physiologic regulation of food intake in response to high-fat feeding. |
format | Online Article Text |
id | pubmed-4314532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-43145322015-02-14 Evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice Buckman, Laura B. Thompson, Misty M. Lippert, Rachel N. Blackwell, Timothy S. Yull, Fiona E. Ellacott, Kate L.J. Mol Metab Brief Communication OBJECTIVE: Introduction of a high-fat diet to mice results in a period of voracious feeding, known as hyperphagia, before homeostatic mechanisms prevail to restore energy intake to an isocaloric level. Acute high-fat diet hyperphagia induces astrocyte activation in the rodent hypothalamus, suggesting a potential role of these cells in the homeostatic response to the diet. The objective of this study was to determine physiologic role of astrocytes in the acute homeostatic response to high-fat feeding. METHODS: We bred a transgenic mouse model with doxycycline-inducible inhibition of NFkappaB (NFκB) signaling in astrocytes to determine the effect of loss of NFκB-mediated astrocyte activation on acute high-fat hyperphagia. ELISA was used to measure the levels of markers of astrocyte activation, glial-fibrillary acidic protein (GFAP) and S100B, in the medial basal hypothalamus. RESULTS: Inhibition of NFκB signaling in astrocytes prevented acute high-fat diet-induced astrocyte activation and resulted in a 15% increase in caloric intake (P < 0.01) in the first 24 h after introduction of the diet. CONCLUSIONS: These data reveal a novel homeostatic role for astrocytes in the acute physiologic regulation of food intake in response to high-fat feeding. Elsevier 2014-10-16 /pmc/articles/PMC4314532/ /pubmed/25685690 http://dx.doi.org/10.1016/j.molmet.2014.10.001 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Brief Communication Buckman, Laura B. Thompson, Misty M. Lippert, Rachel N. Blackwell, Timothy S. Yull, Fiona E. Ellacott, Kate L.J. Evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice |
title | Evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice |
title_full | Evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice |
title_fullStr | Evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice |
title_full_unstemmed | Evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice |
title_short | Evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice |
title_sort | evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314532/ https://www.ncbi.nlm.nih.gov/pubmed/25685690 http://dx.doi.org/10.1016/j.molmet.2014.10.001 |
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