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Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy
BACKGROUND: Alveoli, the milk-producing units of the mammary gland, are generated during pregnancy by collaboration of different epithelial cell types. We present the first analysis of transcriptional changes within the hormone sensing population during pregnancy. Hormone-receptor positive (HR+) cel...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314744/ https://www.ncbi.nlm.nih.gov/pubmed/25623114 http://dx.doi.org/10.1186/s12861-015-0058-9 |
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author | De Silva, Duvini Kunasegaran, Kamini Ghosh, Sujoy Pietersen, Alexandra M |
author_facet | De Silva, Duvini Kunasegaran, Kamini Ghosh, Sujoy Pietersen, Alexandra M |
author_sort | De Silva, Duvini |
collection | PubMed |
description | BACKGROUND: Alveoli, the milk-producing units of the mammary gland, are generated during pregnancy by collaboration of different epithelial cell types. We present the first analysis of transcriptional changes within the hormone sensing population during pregnancy. Hormone-receptor positive (HR+) cells play a key role in the initiation of alveologenesis as they sense systemic hormonal changes and translate these into local instructions for neighboring HR- cells. We recently showed that IGF2 is produced specifically by HR+ cells in early pregnancy, but is undetectable in the virgin state. Here, we define the transcriptome of HR+ cells in early pregnancy with the aim to elucidate additional changes that are unique for this dynamic developmental time window. RESULTS: We harvested mammary glands from virgin, 3-day and 7-day pregnant mice and isolated a few hundred hormone-sensing cells per animal by FACS for microarray analysis. There was a high concordance between animals with a clear induction of cell cycle progression genes at day 3 of pregnancy and molecules involved in paracrine signalling at day 7. CONCLUSIONS: These findings underscore the proliferative capacity of HR+ cells upon specific stimuli and elucidate developmentally-restricted changes in cellular communication. Since the majority of breast cancers are HR+, with a variable proportion of HR+ cells per tumor, we anticipate that this data set will aid further studies into the regulation of HR+ cell proliferation and the role of heterotypic signalling within tumors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12861-015-0058-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4314744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43147442015-02-04 Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy De Silva, Duvini Kunasegaran, Kamini Ghosh, Sujoy Pietersen, Alexandra M BMC Dev Biol Research Article BACKGROUND: Alveoli, the milk-producing units of the mammary gland, are generated during pregnancy by collaboration of different epithelial cell types. We present the first analysis of transcriptional changes within the hormone sensing population during pregnancy. Hormone-receptor positive (HR+) cells play a key role in the initiation of alveologenesis as they sense systemic hormonal changes and translate these into local instructions for neighboring HR- cells. We recently showed that IGF2 is produced specifically by HR+ cells in early pregnancy, but is undetectable in the virgin state. Here, we define the transcriptome of HR+ cells in early pregnancy with the aim to elucidate additional changes that are unique for this dynamic developmental time window. RESULTS: We harvested mammary glands from virgin, 3-day and 7-day pregnant mice and isolated a few hundred hormone-sensing cells per animal by FACS for microarray analysis. There was a high concordance between animals with a clear induction of cell cycle progression genes at day 3 of pregnancy and molecules involved in paracrine signalling at day 7. CONCLUSIONS: These findings underscore the proliferative capacity of HR+ cells upon specific stimuli and elucidate developmentally-restricted changes in cellular communication. Since the majority of breast cancers are HR+, with a variable proportion of HR+ cells per tumor, we anticipate that this data set will aid further studies into the regulation of HR+ cell proliferation and the role of heterotypic signalling within tumors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12861-015-0058-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-01-27 /pmc/articles/PMC4314744/ /pubmed/25623114 http://dx.doi.org/10.1186/s12861-015-0058-9 Text en © De Silva et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article De Silva, Duvini Kunasegaran, Kamini Ghosh, Sujoy Pietersen, Alexandra M Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy |
title | Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy |
title_full | Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy |
title_fullStr | Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy |
title_full_unstemmed | Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy |
title_short | Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy |
title_sort | transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314744/ https://www.ncbi.nlm.nih.gov/pubmed/25623114 http://dx.doi.org/10.1186/s12861-015-0058-9 |
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