Cargando…

Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line

Pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. It is characterized by a poor diagnosis and lack of response to conventional therapy. Recent studies have suggested that disulfiram (DSF), a member of the d...

Descripción completa

Detalles Bibliográficos
Autores principales: Dastjerdi, M. Nikbakht, Babazadeh, Z., Rabbani, M., Gharagozloo, M., Esmaeili, A., Narimani, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314877/
https://www.ncbi.nlm.nih.gov/pubmed/25657800
_version_ 1782355386548879360
author Dastjerdi, M. Nikbakht
Babazadeh, Z.
Rabbani, M.
Gharagozloo, M.
Esmaeili, A.
Narimani, M.
author_facet Dastjerdi, M. Nikbakht
Babazadeh, Z.
Rabbani, M.
Gharagozloo, M.
Esmaeili, A.
Narimani, M.
author_sort Dastjerdi, M. Nikbakht
collection PubMed
description Pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. It is characterized by a poor diagnosis and lack of response to conventional therapy. Recent studies have suggested that disulfiram (DSF), a member of the dithiocarbamate family, may have antitumor activity. This study aimed to evaluate the in vitro effect of DSF on apoptosis in human pancreatic cancerous cell line (PANC-1). PANC-1 cells were cultured and treated with DSF at doses of 5, 10, 13 μM for 24 h and apoptosis was measured. Methylation specific PCR (MS-PCR) and real-time quantitative PCR were carried out to detect the methylation pattern and to estimate the mRNA expression levels of RASSF1A, p21 and Bax. MS-PCR analysis demonstrated that no unmethylated band was apeared in PANC-1 cell line after DSF treatments. The real-time quantitative PCR results showed no significant mRNA expression for RASSF1A (p>0.05); whereas p21 and Bax expression were significantly (p<0.01) enhanced after treatment with DSF. The results of the current study indicated that DSF can induce appoptosis in PANC-1 through p21 and Bax pathway but not through RASSF1A.
format Online
Article
Text
id pubmed-4314877
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-43148772015-02-05 Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line Dastjerdi, M. Nikbakht Babazadeh, Z. Rabbani, M. Gharagozloo, M. Esmaeili, A. Narimani, M. Res Pharm Sci Original Article Pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. It is characterized by a poor diagnosis and lack of response to conventional therapy. Recent studies have suggested that disulfiram (DSF), a member of the dithiocarbamate family, may have antitumor activity. This study aimed to evaluate the in vitro effect of DSF on apoptosis in human pancreatic cancerous cell line (PANC-1). PANC-1 cells were cultured and treated with DSF at doses of 5, 10, 13 μM for 24 h and apoptosis was measured. Methylation specific PCR (MS-PCR) and real-time quantitative PCR were carried out to detect the methylation pattern and to estimate the mRNA expression levels of RASSF1A, p21 and Bax. MS-PCR analysis demonstrated that no unmethylated band was apeared in PANC-1 cell line after DSF treatments. The real-time quantitative PCR results showed no significant mRNA expression for RASSF1A (p>0.05); whereas p21 and Bax expression were significantly (p<0.01) enhanced after treatment with DSF. The results of the current study indicated that DSF can induce appoptosis in PANC-1 through p21 and Bax pathway but not through RASSF1A. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4314877/ /pubmed/25657800 Text en Copyright: © Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Dastjerdi, M. Nikbakht
Babazadeh, Z.
Rabbani, M.
Gharagozloo, M.
Esmaeili, A.
Narimani, M.
Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line
title Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line
title_full Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line
title_fullStr Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line
title_full_unstemmed Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line
title_short Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line
title_sort effects of disulfiram on apoptosis in panc-1 human pancreatic cancer cell line
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314877/
https://www.ncbi.nlm.nih.gov/pubmed/25657800
work_keys_str_mv AT dastjerdimnikbakht effectsofdisulfiramonapoptosisinpanc1humanpancreaticcancercellline
AT babazadehz effectsofdisulfiramonapoptosisinpanc1humanpancreaticcancercellline
AT rabbanim effectsofdisulfiramonapoptosisinpanc1humanpancreaticcancercellline
AT gharagozloom effectsofdisulfiramonapoptosisinpanc1humanpancreaticcancercellline
AT esmaeilia effectsofdisulfiramonapoptosisinpanc1humanpancreaticcancercellline
AT narimanim effectsofdisulfiramonapoptosisinpanc1humanpancreaticcancercellline