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Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line
Pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. It is characterized by a poor diagnosis and lack of response to conventional therapy. Recent studies have suggested that disulfiram (DSF), a member of the d...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314877/ https://www.ncbi.nlm.nih.gov/pubmed/25657800 |
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author | Dastjerdi, M. Nikbakht Babazadeh, Z. Rabbani, M. Gharagozloo, M. Esmaeili, A. Narimani, M. |
author_facet | Dastjerdi, M. Nikbakht Babazadeh, Z. Rabbani, M. Gharagozloo, M. Esmaeili, A. Narimani, M. |
author_sort | Dastjerdi, M. Nikbakht |
collection | PubMed |
description | Pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. It is characterized by a poor diagnosis and lack of response to conventional therapy. Recent studies have suggested that disulfiram (DSF), a member of the dithiocarbamate family, may have antitumor activity. This study aimed to evaluate the in vitro effect of DSF on apoptosis in human pancreatic cancerous cell line (PANC-1). PANC-1 cells were cultured and treated with DSF at doses of 5, 10, 13 μM for 24 h and apoptosis was measured. Methylation specific PCR (MS-PCR) and real-time quantitative PCR were carried out to detect the methylation pattern and to estimate the mRNA expression levels of RASSF1A, p21 and Bax. MS-PCR analysis demonstrated that no unmethylated band was apeared in PANC-1 cell line after DSF treatments. The real-time quantitative PCR results showed no significant mRNA expression for RASSF1A (p>0.05); whereas p21 and Bax expression were significantly (p<0.01) enhanced after treatment with DSF. The results of the current study indicated that DSF can induce appoptosis in PANC-1 through p21 and Bax pathway but not through RASSF1A. |
format | Online Article Text |
id | pubmed-4314877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-43148772015-02-05 Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line Dastjerdi, M. Nikbakht Babazadeh, Z. Rabbani, M. Gharagozloo, M. Esmaeili, A. Narimani, M. Res Pharm Sci Original Article Pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. It is characterized by a poor diagnosis and lack of response to conventional therapy. Recent studies have suggested that disulfiram (DSF), a member of the dithiocarbamate family, may have antitumor activity. This study aimed to evaluate the in vitro effect of DSF on apoptosis in human pancreatic cancerous cell line (PANC-1). PANC-1 cells were cultured and treated with DSF at doses of 5, 10, 13 μM for 24 h and apoptosis was measured. Methylation specific PCR (MS-PCR) and real-time quantitative PCR were carried out to detect the methylation pattern and to estimate the mRNA expression levels of RASSF1A, p21 and Bax. MS-PCR analysis demonstrated that no unmethylated band was apeared in PANC-1 cell line after DSF treatments. The real-time quantitative PCR results showed no significant mRNA expression for RASSF1A (p>0.05); whereas p21 and Bax expression were significantly (p<0.01) enhanced after treatment with DSF. The results of the current study indicated that DSF can induce appoptosis in PANC-1 through p21 and Bax pathway but not through RASSF1A. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4314877/ /pubmed/25657800 Text en Copyright: © Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Dastjerdi, M. Nikbakht Babazadeh, Z. Rabbani, M. Gharagozloo, M. Esmaeili, A. Narimani, M. Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line |
title | Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line |
title_full | Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line |
title_fullStr | Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line |
title_full_unstemmed | Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line |
title_short | Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line |
title_sort | effects of disulfiram on apoptosis in panc-1 human pancreatic cancer cell line |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314877/ https://www.ncbi.nlm.nih.gov/pubmed/25657800 |
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