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Effect of metformin on insulin-resistant endothelial cell function
The aim of the present study was to investigate the effect of metformin on the function of insulin-resistant (IR) endothelial cells. A model of IR endothelial cells was established by incubating cells with 30 mM glucose, 1 μM dexamethasone and various concentrations of insulin. The nitric oxide (NO)...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314968/ https://www.ncbi.nlm.nih.gov/pubmed/25663871 http://dx.doi.org/10.3892/ol.2015.2883 |
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author | CHEN, HAIYAN LI, JIE YANG, OU KONG, JIAN LIN, GUANGZHU |
author_facet | CHEN, HAIYAN LI, JIE YANG, OU KONG, JIAN LIN, GUANGZHU |
author_sort | CHEN, HAIYAN |
collection | PubMed |
description | The aim of the present study was to investigate the effect of metformin on the function of insulin-resistant (IR) endothelial cells. A model of IR endothelial cells was established by incubating cells with 30 mM glucose, 1 μM dexamethasone and various concentrations of insulin. The nitric oxide (NO) content of the endothelial cells was determined by measuring the rate of nitroreductase production; the endothelin (ET) concentration was examined by enzyme-linked immunosorbent assay; and the expression levels of endothelial nitric oxide synthase (eNOS) were detected using western blotting. The optimal conditions for inducing insulin resistance in endothelial cells were a combination treatment of 10(−4) mmol/l insulin, 30 mM glucose and 1 μM dexamethasone for 48 h. Notably, metformin administration significantly increased the NO content and reduced the ET-1 concentration in the IR cells compared with the non-treated control cells (P<0.05); furthermore, metformin significantly increased the intracellular eNOS protein expression in IR endothelial cells compared with the non-treated control cells (P<0.05), with an optimal metformin concentration of 10(−3) mmol/l. Thus, the present study identified that metformin improves the function of IR endothelial cells, possibly through promoting eNOS protein expression and increasing the NO content. |
format | Online Article Text |
id | pubmed-4314968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-43149682015-02-06 Effect of metformin on insulin-resistant endothelial cell function CHEN, HAIYAN LI, JIE YANG, OU KONG, JIAN LIN, GUANGZHU Oncol Lett Articles The aim of the present study was to investigate the effect of metformin on the function of insulin-resistant (IR) endothelial cells. A model of IR endothelial cells was established by incubating cells with 30 mM glucose, 1 μM dexamethasone and various concentrations of insulin. The nitric oxide (NO) content of the endothelial cells was determined by measuring the rate of nitroreductase production; the endothelin (ET) concentration was examined by enzyme-linked immunosorbent assay; and the expression levels of endothelial nitric oxide synthase (eNOS) were detected using western blotting. The optimal conditions for inducing insulin resistance in endothelial cells were a combination treatment of 10(−4) mmol/l insulin, 30 mM glucose and 1 μM dexamethasone for 48 h. Notably, metformin administration significantly increased the NO content and reduced the ET-1 concentration in the IR cells compared with the non-treated control cells (P<0.05); furthermore, metformin significantly increased the intracellular eNOS protein expression in IR endothelial cells compared with the non-treated control cells (P<0.05), with an optimal metformin concentration of 10(−3) mmol/l. Thus, the present study identified that metformin improves the function of IR endothelial cells, possibly through promoting eNOS protein expression and increasing the NO content. D.A. Spandidos 2015-03 2015-01-16 /pmc/articles/PMC4314968/ /pubmed/25663871 http://dx.doi.org/10.3892/ol.2015.2883 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles CHEN, HAIYAN LI, JIE YANG, OU KONG, JIAN LIN, GUANGZHU Effect of metformin on insulin-resistant endothelial cell function |
title | Effect of metformin on insulin-resistant endothelial cell function |
title_full | Effect of metformin on insulin-resistant endothelial cell function |
title_fullStr | Effect of metformin on insulin-resistant endothelial cell function |
title_full_unstemmed | Effect of metformin on insulin-resistant endothelial cell function |
title_short | Effect of metformin on insulin-resistant endothelial cell function |
title_sort | effect of metformin on insulin-resistant endothelial cell function |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314968/ https://www.ncbi.nlm.nih.gov/pubmed/25663871 http://dx.doi.org/10.3892/ol.2015.2883 |
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