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Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films
Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315565/ https://www.ncbi.nlm.nih.gov/pubmed/25670900 http://dx.doi.org/10.2147/IJN.S73628 |
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author | Liskova, Jana Babchenko, Oleg Varga, Marian Kromka, Alexander Hadraba, Daniel Svindrych, Zdenek Burdikova, Zuzana Bacakova, Lucie |
author_facet | Liskova, Jana Babchenko, Oleg Varga, Marian Kromka, Alexander Hadraba, Daniel Svindrych, Zdenek Burdikova, Zuzana Bacakova, Lucie |
author_sort | Liskova, Jana |
collection | PubMed |
description | Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings. |
format | Online Article Text |
id | pubmed-4315565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43155652015-02-10 Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films Liskova, Jana Babchenko, Oleg Varga, Marian Kromka, Alexander Hadraba, Daniel Svindrych, Zdenek Burdikova, Zuzana Bacakova, Lucie Int J Nanomedicine Original Research Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings. Dove Medical Press 2015-01-27 /pmc/articles/PMC4315565/ /pubmed/25670900 http://dx.doi.org/10.2147/IJN.S73628 Text en © 2015 Liskova et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Liskova, Jana Babchenko, Oleg Varga, Marian Kromka, Alexander Hadraba, Daniel Svindrych, Zdenek Burdikova, Zuzana Bacakova, Lucie Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films |
title | Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films |
title_full | Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films |
title_fullStr | Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films |
title_full_unstemmed | Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films |
title_short | Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films |
title_sort | osteogenic cell differentiation on h-terminated and o-terminated nanocrystalline diamond films |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315565/ https://www.ncbi.nlm.nih.gov/pubmed/25670900 http://dx.doi.org/10.2147/IJN.S73628 |
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