Cargando…

Combined Analysis of the Tobacco Metabolites Cotinine and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Human Urine

[Image: see text] Two of the most widely measured compounds in the urine of people who use tobacco products are cotinine, a major metabolite of the addictive constituent nicotine, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), a metabolite of the powerful lung carcinogen 4-(methylnitrosam...

Descripción completa

Detalles Bibliográficos
Autores principales: Kotandeniya, Delshanee, Carmella, Steven G., Ming, Xun, Murphy, Sharon E., Hecht, Stephen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315695/
https://www.ncbi.nlm.nih.gov/pubmed/25544129
http://dx.doi.org/10.1021/ac504047j
_version_ 1782355513479004160
author Kotandeniya, Delshanee
Carmella, Steven G.
Ming, Xun
Murphy, Sharon E.
Hecht, Stephen S.
author_facet Kotandeniya, Delshanee
Carmella, Steven G.
Ming, Xun
Murphy, Sharon E.
Hecht, Stephen S.
author_sort Kotandeniya, Delshanee
collection PubMed
description [Image: see text] Two of the most widely measured compounds in the urine of people who use tobacco products are cotinine, a major metabolite of the addictive constituent nicotine, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), a metabolite of the powerful lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Thousands of analyses have been reported in the literature, carried out exclusively, to the best of our knowledge, by separate methods. In the study reported here, we have developed a sensitive, accurate, and precise liquid chromatography-electrospray ionization-tandem mass spectrometry-selected reaction monitoring method for the combined analysis of total cotinine (the sum of cotinine and its glucuronide) and total NNAL (the sum of NNAL and its glucuronide). The new method quantifies naturally occurring [(13)C]cotinine to minimize problems associated with the vast differences in concentration of total cotinine and total NNAL in urine. This method should greatly facilitate future determinations of these important compounds.
format Online
Article
Text
id pubmed-4315695
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-43156952015-02-04 Combined Analysis of the Tobacco Metabolites Cotinine and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Human Urine Kotandeniya, Delshanee Carmella, Steven G. Ming, Xun Murphy, Sharon E. Hecht, Stephen S. Anal Chem [Image: see text] Two of the most widely measured compounds in the urine of people who use tobacco products are cotinine, a major metabolite of the addictive constituent nicotine, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), a metabolite of the powerful lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Thousands of analyses have been reported in the literature, carried out exclusively, to the best of our knowledge, by separate methods. In the study reported here, we have developed a sensitive, accurate, and precise liquid chromatography-electrospray ionization-tandem mass spectrometry-selected reaction monitoring method for the combined analysis of total cotinine (the sum of cotinine and its glucuronide) and total NNAL (the sum of NNAL and its glucuronide). The new method quantifies naturally occurring [(13)C]cotinine to minimize problems associated with the vast differences in concentration of total cotinine and total NNAL in urine. This method should greatly facilitate future determinations of these important compounds. American Chemical Society 2014-12-28 2015-02-03 /pmc/articles/PMC4315695/ /pubmed/25544129 http://dx.doi.org/10.1021/ac504047j Text en Copyright © 2014 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Kotandeniya, Delshanee
Carmella, Steven G.
Ming, Xun
Murphy, Sharon E.
Hecht, Stephen S.
Combined Analysis of the Tobacco Metabolites Cotinine and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Human Urine
title Combined Analysis of the Tobacco Metabolites Cotinine and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Human Urine
title_full Combined Analysis of the Tobacco Metabolites Cotinine and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Human Urine
title_fullStr Combined Analysis of the Tobacco Metabolites Cotinine and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Human Urine
title_full_unstemmed Combined Analysis of the Tobacco Metabolites Cotinine and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Human Urine
title_short Combined Analysis of the Tobacco Metabolites Cotinine and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Human Urine
title_sort combined analysis of the tobacco metabolites cotinine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human urine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315695/
https://www.ncbi.nlm.nih.gov/pubmed/25544129
http://dx.doi.org/10.1021/ac504047j
work_keys_str_mv AT kotandeniyadelshanee combinedanalysisofthetobaccometabolitescotinineand4methylnitrosamino13pyridyl1butanolinhumanurine
AT carmellasteveng combinedanalysisofthetobaccometabolitescotinineand4methylnitrosamino13pyridyl1butanolinhumanurine
AT mingxun combinedanalysisofthetobaccometabolitescotinineand4methylnitrosamino13pyridyl1butanolinhumanurine
AT murphysharone combinedanalysisofthetobaccometabolitescotinineand4methylnitrosamino13pyridyl1butanolinhumanurine
AT hechtstephens combinedanalysisofthetobaccometabolitescotinineand4methylnitrosamino13pyridyl1butanolinhumanurine