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Characterization of Monobody Scaffold Interactions with Ligand via Force Spectroscopy and Steered Molecular Dynamics

Monobodies are antibody alternatives derived from fibronectin that are thermodynamically stable, small in size, and can be produced in bacterial systems. Monobodies have been engineered to bind a wide variety of target proteins with high affinity and specificity. Using alanine-scanning mutagenesis s...

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Detalles Bibliográficos
Autores principales:	Cheung, Luthur Siu-Lun, Shea, Daniel J., Nicholes, Nathan, Date, Amol, Ostermeier, Marc, Konstantopoulos, Konstantinos
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4316159/ https://www.ncbi.nlm.nih.gov/pubmed/25650239 http://dx.doi.org/10.1038/srep08247

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