Evaluation of the Performances of Ribosomal Database Project (RDP) Classifier for Taxonomic Assignment of 16S rRNA Metabarcoding Sequences Generated from Illumina-Solexa NGS

Here we report a benchmark of the effect of bootstrap cut-off values of the RDP Classifier tool in terms of data retention along the different taxonomic ranks by using Illumina reads. Results provide guidelines for planning sequencing depths and selection of bootstrap cut-off in taxonomic assignment...

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Detalles Bibliográficos
Autores principales: Bacci, Giovanni, Bani, Alessia, Bazzicalupo, Marco, Ceccherini, Maria Teresa, Galardini, Marco, Nannipieri, Paolo, Pietramellara, Giacomo, Mengoni, Alessio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2015
Materias:
Short Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4316179/
https://www.ncbi.nlm.nih.gov/pubmed/25653722
http://dx.doi.org/10.7150/jgen.9204
Descripción
Sumario:Here we report a benchmark of the effect of bootstrap cut-off values of the RDP Classifier tool in terms of data retention along the different taxonomic ranks by using Illumina reads. Results provide guidelines for planning sequencing depths and selection of bootstrap cut-off in taxonomic assignments.

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