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Expression and significance of NELIN and SM22α in varicose vein tissue

The aim of the present study was to investigate the expression of NELIN and SM22α in lower extremity varicose vein tissue, and their association with varicose veins. Tissue samples were collected from 18 patients with lower extremity varicose veins for the experimental group, while normal great saph...

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Autores principales: CHEN, SHIHUI, QIN, SHIYONG, WANG, MINGHAI, ZHANG, SHUGUANG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4316901/
https://www.ncbi.nlm.nih.gov/pubmed/25667639
http://dx.doi.org/10.3892/etm.2015.2170
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author CHEN, SHIHUI
QIN, SHIYONG
WANG, MINGHAI
ZHANG, SHUGUANG
author_facet CHEN, SHIHUI
QIN, SHIYONG
WANG, MINGHAI
ZHANG, SHUGUANG
author_sort CHEN, SHIHUI
collection PubMed
description The aim of the present study was to investigate the expression of NELIN and SM22α in lower extremity varicose vein tissue, and their association with varicose veins. Tissue samples were collected from 18 patients with lower extremity varicose veins for the experimental group, while normal great saphenous vein tissue was reserved during coronary artery bypass surgery from 14 patients for the controls. Reverse transcription polymerase chain reaction (RT-PCR) analysis was applied to detect the mRNA expression levels of NELIN and SM22α, while immunohistochemical techniques were used to detect the protein expression levels in the normal and abnormal veins. RT-PCR results revealed that the mRNA expression levels of NELIN and SM22α in the experimental group decreased significantly when compared with the control group (P<0.01). In the two groups, immunohistochemical staining demonstrated that NELIN and SM22α were primarily expressed in the cytoplasm of smooth muscle cells, and the expression quantity decreased significantly in the experimental group when compared with the control group (P<0.05). The low expression of SM22α in the primary lower limb varicose vein tissue indicated that the vascular smooth muscle cell layer had transformed from a contractile to a secretory phenotype, which may have resulted in the remodeling of the vein walls and the occurrence of varicose veins. Therefore, NELIN and SM22α were demonstrated to play a key role in the development of varicosity.
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spelling pubmed-43169012015-02-09 Expression and significance of NELIN and SM22α in varicose vein tissue CHEN, SHIHUI QIN, SHIYONG WANG, MINGHAI ZHANG, SHUGUANG Exp Ther Med Articles The aim of the present study was to investigate the expression of NELIN and SM22α in lower extremity varicose vein tissue, and their association with varicose veins. Tissue samples were collected from 18 patients with lower extremity varicose veins for the experimental group, while normal great saphenous vein tissue was reserved during coronary artery bypass surgery from 14 patients for the controls. Reverse transcription polymerase chain reaction (RT-PCR) analysis was applied to detect the mRNA expression levels of NELIN and SM22α, while immunohistochemical techniques were used to detect the protein expression levels in the normal and abnormal veins. RT-PCR results revealed that the mRNA expression levels of NELIN and SM22α in the experimental group decreased significantly when compared with the control group (P<0.01). In the two groups, immunohistochemical staining demonstrated that NELIN and SM22α were primarily expressed in the cytoplasm of smooth muscle cells, and the expression quantity decreased significantly in the experimental group when compared with the control group (P<0.05). The low expression of SM22α in the primary lower limb varicose vein tissue indicated that the vascular smooth muscle cell layer had transformed from a contractile to a secretory phenotype, which may have resulted in the remodeling of the vein walls and the occurrence of varicose veins. Therefore, NELIN and SM22α were demonstrated to play a key role in the development of varicosity. D.A. Spandidos 2015-03 2015-01-08 /pmc/articles/PMC4316901/ /pubmed/25667639 http://dx.doi.org/10.3892/etm.2015.2170 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
CHEN, SHIHUI
QIN, SHIYONG
WANG, MINGHAI
ZHANG, SHUGUANG
Expression and significance of NELIN and SM22α in varicose vein tissue
title Expression and significance of NELIN and SM22α in varicose vein tissue
title_full Expression and significance of NELIN and SM22α in varicose vein tissue
title_fullStr Expression and significance of NELIN and SM22α in varicose vein tissue
title_full_unstemmed Expression and significance of NELIN and SM22α in varicose vein tissue
title_short Expression and significance of NELIN and SM22α in varicose vein tissue
title_sort expression and significance of nelin and sm22α in varicose vein tissue
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4316901/
https://www.ncbi.nlm.nih.gov/pubmed/25667639
http://dx.doi.org/10.3892/etm.2015.2170
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