Green tea extract therapy diminishes hepatic fibrosis mediated by dual exposure to carbon tetrachloride and ethanol: A histopathological study

The aim of the present study was to investigate the hepatoprotective effect of green tea extract (GTE) against the hepatic fibrosis induced by carbon tetrachloride (CCl(4)), ethanol, and dual exposure to CCl(4) plus ethanol in rats. In particular, an investigation of the three-dimensional architecture was conducted using scanning electron microscopy. Various techniques revealed that hepatic fibrosis with intermingled fibers was located between cells in the CCl(4), ethanol and combined CCl(4) plus ethanol groups. The hepatic fibrosis differed among the ethanol, CCl(4) and CCl(4) plus ethanol groups in terms of the type, thickness and distribution of fibers. The fibrotic lesions virtually disappeared in all the groups after 25 days of treatment with GTE, returning the architecture of the liver tissue to its normal status. The rats were also found to regain normal body weight and fur color, which had earlier been discolored due to weight loss. The autopsy results also showed that the animal livers returned to the normal shape and color. GTE demonstrated the same clear action in attenuating the hepatofibrosis for all three inducing treatments, by impairing collagen fibers, eliminating lipid peroxidation and returning the liver architecture to normal. GTE presents a safe therapeutic strategy for hepatic fibrosis.