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Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression

Bushen Zhuangjin Decoction (BZD), a well-known formulation in Traditional Chinese Medicine, has been widely used for the treatment of osteoarthritis (OA). Due to the poor intrinsic repair capacity of chondrocytes, promoting the proliferation of chondrocytes is an efficient treatment to delay the pro...

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Autores principales: LI, XIHAI, CHEN, JIASHOU, LIANG, WENNA, LI, HUITING, LIU, FAYUAN, WENG, XIAPING, LIN, PINGDONG, CHEN, WENLIE, ZHENG, CHUNSONG, XU, HUIFENG, LIU, XIANXIANG, YE, HONGZHI
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4316974/
https://www.ncbi.nlm.nih.gov/pubmed/25667638
http://dx.doi.org/10.3892/etm.2015.2214
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author LI, XIHAI
CHEN, JIASHOU
LIANG, WENNA
LI, HUITING
LIU, FAYUAN
WENG, XIAPING
LIN, PINGDONG
CHEN, WENLIE
ZHENG, CHUNSONG
XU, HUIFENG
LIU, XIANXIANG
YE, HONGZHI
author_facet LI, XIHAI
CHEN, JIASHOU
LIANG, WENNA
LI, HUITING
LIU, FAYUAN
WENG, XIAPING
LIN, PINGDONG
CHEN, WENLIE
ZHENG, CHUNSONG
XU, HUIFENG
LIU, XIANXIANG
YE, HONGZHI
author_sort LI, XIHAI
collection PubMed
description Bushen Zhuangjin Decoction (BZD), a well-known formulation in Traditional Chinese Medicine, has been widely used for the treatment of osteoarthritis (OA). Due to the poor intrinsic repair capacity of chondrocytes, promoting the proliferation of chondrocytes is an efficient treatment to delay the progression of cartilage degradation. The present study, therefore, focused on the effect of BZD on chondrocyte proliferation, exploring the mechanism of BZD on the inhibition of cartilage degradation. Chondrocytes isolated from the knee articular cartilage of Sprague Dawley rats were cultured and identified by type II collagen immunohistochemistry. It was found that BZD promoted chondrocyte viability in a dose- and time-dependent manner. To investigate if BZD promoted the chondrocyte viability by stimulating the cell cycle progression a flow cytometer was used, and the results showed that the percentage proportion of G0/G1 cells was significantly lower, and the percentage proportion of S cells was significantly higher, in treated cells compared with that in untreated cells. To gain insight into the mechanism underlying the effect of BZD on the cell cycle progression, the mRNA and protein expression of cyclin D1, cyclin-dependent kinase 4 (CDK4), CDK6 and p21 was measured by reverse transcription-polymerase chain reaction and western blotting, respectively. The mRNA and protein expression of cyclin D1, CDK4 and CDK6 in the BZD-treated chondrocytes was significantly upregulated, while the mRNA and protein expression of p21 was significantly downregulated, compared with that in the untreated chondrocytes. These results suggested that BZD promoted chondrocyte proliferation by accelerating G1/S transition, indicating that BZD is a potential therapeutic agent for the treatment of OA.
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spelling pubmed-43169742015-02-09 Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression LI, XIHAI CHEN, JIASHOU LIANG, WENNA LI, HUITING LIU, FAYUAN WENG, XIAPING LIN, PINGDONG CHEN, WENLIE ZHENG, CHUNSONG XU, HUIFENG LIU, XIANXIANG YE, HONGZHI Exp Ther Med Articles Bushen Zhuangjin Decoction (BZD), a well-known formulation in Traditional Chinese Medicine, has been widely used for the treatment of osteoarthritis (OA). Due to the poor intrinsic repair capacity of chondrocytes, promoting the proliferation of chondrocytes is an efficient treatment to delay the progression of cartilage degradation. The present study, therefore, focused on the effect of BZD on chondrocyte proliferation, exploring the mechanism of BZD on the inhibition of cartilage degradation. Chondrocytes isolated from the knee articular cartilage of Sprague Dawley rats were cultured and identified by type II collagen immunohistochemistry. It was found that BZD promoted chondrocyte viability in a dose- and time-dependent manner. To investigate if BZD promoted the chondrocyte viability by stimulating the cell cycle progression a flow cytometer was used, and the results showed that the percentage proportion of G0/G1 cells was significantly lower, and the percentage proportion of S cells was significantly higher, in treated cells compared with that in untreated cells. To gain insight into the mechanism underlying the effect of BZD on the cell cycle progression, the mRNA and protein expression of cyclin D1, cyclin-dependent kinase 4 (CDK4), CDK6 and p21 was measured by reverse transcription-polymerase chain reaction and western blotting, respectively. The mRNA and protein expression of cyclin D1, CDK4 and CDK6 in the BZD-treated chondrocytes was significantly upregulated, while the mRNA and protein expression of p21 was significantly downregulated, compared with that in the untreated chondrocytes. These results suggested that BZD promoted chondrocyte proliferation by accelerating G1/S transition, indicating that BZD is a potential therapeutic agent for the treatment of OA. D.A. Spandidos 2015-03 2015-01-23 /pmc/articles/PMC4316974/ /pubmed/25667638 http://dx.doi.org/10.3892/etm.2015.2214 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
LI, XIHAI
CHEN, JIASHOU
LIANG, WENNA
LI, HUITING
LIU, FAYUAN
WENG, XIAPING
LIN, PINGDONG
CHEN, WENLIE
ZHENG, CHUNSONG
XU, HUIFENG
LIU, XIANXIANG
YE, HONGZHI
Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression
title Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression
title_full Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression
title_fullStr Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression
title_full_unstemmed Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression
title_short Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression
title_sort bushen zhuangjin decoction promotes chondrocyte proliferation by stimulating cell cycle progression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4316974/
https://www.ncbi.nlm.nih.gov/pubmed/25667638
http://dx.doi.org/10.3892/etm.2015.2214
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