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Improvement in nutrient handling in STZ induced diabetic rats treated with Ocimum gratissimum

OBJECTIVE: Alteration in digestive and absorptive enzymatic activities has been reported in diabetes mellitus (DM), but not with Ocimum gratissimum (OG) treatment. This study was, therefore, designed to indirectly assess the effect of DM and treatment with OG on nutrient digestion and absorption, th...

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Autores principales: Okon, Uduak Akpan, Davies, Koofreh Godwin, Olubobokun, Titilope Helen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318102/
https://www.ncbi.nlm.nih.gov/pubmed/25664269
http://dx.doi.org/10.4103/2229-516X.149242
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author Okon, Uduak Akpan
Davies, Koofreh Godwin
Olubobokun, Titilope Helen
author_facet Okon, Uduak Akpan
Davies, Koofreh Godwin
Olubobokun, Titilope Helen
author_sort Okon, Uduak Akpan
collection PubMed
description OBJECTIVE: Alteration in digestive and absorptive enzymatic activities has been reported in diabetes mellitus (DM), but not with Ocimum gratissimum (OG) treatment. This study was, therefore, designed to indirectly assess the effect of DM and treatment with OG on nutrient digestion and absorption, through estimation of their fecal excretion. MATERIALS AND METHODS: Animals were randomly assigned into three groups of six per group for control, DM and diabetic mellitus treated (DMT). Diabetes was induced by single intraperitoneal injection of 65 mg/kg streptozotocin in the test groups. OG was administered to the DMT group at dose of 1500 mg/kg once daily for 28 days. Fecal glucose, protein and cholesterol were determined. RESULTS: Fecal glucose was significantly (P < 0.001) lower in the DM group compared to the control and DMT groups, with the DMT groups significantly (P < 0.001) lower than the control. Fecal protein was significantly (P < 0.001) lower in the DM group than the control whereas it was significantly lower in the DMT groups than the DM. Fecal cholesterol was significantly (P < 0.001) higher in the DM than the DMT and control groups with DMT significantly (P < 0.01) higher than the control. CONCLUSION: This result indicates the propensity of OG to reverse impairment of nutrient digestion and absorption in DM.
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spelling pubmed-43181022015-02-06 Improvement in nutrient handling in STZ induced diabetic rats treated with Ocimum gratissimum Okon, Uduak Akpan Davies, Koofreh Godwin Olubobokun, Titilope Helen Int J Appl Basic Med Res Original Article OBJECTIVE: Alteration in digestive and absorptive enzymatic activities has been reported in diabetes mellitus (DM), but not with Ocimum gratissimum (OG) treatment. This study was, therefore, designed to indirectly assess the effect of DM and treatment with OG on nutrient digestion and absorption, through estimation of their fecal excretion. MATERIALS AND METHODS: Animals were randomly assigned into three groups of six per group for control, DM and diabetic mellitus treated (DMT). Diabetes was induced by single intraperitoneal injection of 65 mg/kg streptozotocin in the test groups. OG was administered to the DMT group at dose of 1500 mg/kg once daily for 28 days. Fecal glucose, protein and cholesterol were determined. RESULTS: Fecal glucose was significantly (P < 0.001) lower in the DM group compared to the control and DMT groups, with the DMT groups significantly (P < 0.001) lower than the control. Fecal protein was significantly (P < 0.001) lower in the DM group than the control whereas it was significantly lower in the DMT groups than the DM. Fecal cholesterol was significantly (P < 0.001) higher in the DM than the DMT and control groups with DMT significantly (P < 0.01) higher than the control. CONCLUSION: This result indicates the propensity of OG to reverse impairment of nutrient digestion and absorption in DM. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4318102/ /pubmed/25664269 http://dx.doi.org/10.4103/2229-516X.149242 Text en Copyright: © International Journal of Applied and Basic Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Okon, Uduak Akpan
Davies, Koofreh Godwin
Olubobokun, Titilope Helen
Improvement in nutrient handling in STZ induced diabetic rats treated with Ocimum gratissimum
title Improvement in nutrient handling in STZ induced diabetic rats treated with Ocimum gratissimum
title_full Improvement in nutrient handling in STZ induced diabetic rats treated with Ocimum gratissimum
title_fullStr Improvement in nutrient handling in STZ induced diabetic rats treated with Ocimum gratissimum
title_full_unstemmed Improvement in nutrient handling in STZ induced diabetic rats treated with Ocimum gratissimum
title_short Improvement in nutrient handling in STZ induced diabetic rats treated with Ocimum gratissimum
title_sort improvement in nutrient handling in stz induced diabetic rats treated with ocimum gratissimum
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318102/
https://www.ncbi.nlm.nih.gov/pubmed/25664269
http://dx.doi.org/10.4103/2229-516X.149242
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