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Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production
BACKGROUND: The bioethanol production system used in Brazil is based on the fermentation of sucrose from sugarcane feedstock by highly adapted strains of the yeast Saccharomyces cerevisiae. Bacterial contaminants present in the distillery environment often produce yeast-bacteria cellular co-aggregat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318157/ https://www.ncbi.nlm.nih.gov/pubmed/25633848 http://dx.doi.org/10.1186/s12934-015-0196-6 |
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author | Carvalho-Netto, Osmar V Carazzolle, Marcelo F Mofatto, Luciana S Teixeira, Paulo JPL Noronha, Melline F Calderón, Luige AL Mieczkowski, Piotr A Argueso, Juan Lucas Pereira, Gonçalo AG |
author_facet | Carvalho-Netto, Osmar V Carazzolle, Marcelo F Mofatto, Luciana S Teixeira, Paulo JPL Noronha, Melline F Calderón, Luige AL Mieczkowski, Piotr A Argueso, Juan Lucas Pereira, Gonçalo AG |
author_sort | Carvalho-Netto, Osmar V |
collection | PubMed |
description | BACKGROUND: The bioethanol production system used in Brazil is based on the fermentation of sucrose from sugarcane feedstock by highly adapted strains of the yeast Saccharomyces cerevisiae. Bacterial contaminants present in the distillery environment often produce yeast-bacteria cellular co-aggregation particles that resemble yeast-yeast cell adhesion (flocculation). The formation of such particles is undesirable because it slows the fermentation kinetics and reduces the overall bioethanol yield. RESULTS: In this study, we investigated the molecular physiology of one of the main S. cerevisiae strains used in Brazilian bioethanol production, PE-2, under two contrasting conditions: typical fermentation, when most yeast cells are in suspension, and co-aggregated fermentation. The transcriptional profile of PE-2 was assessed by RNA-seq during industrial scale fed-batch fermentation. Comparative analysis between the two conditions revealed transcriptional profiles that were differentiated primarily by a deep gene repression in the co-aggregated samples. The data also indicated that Lactobacillus fermentum was likely the main bacterial species responsible for cellular co-aggregation and for the high levels of organic acids detected in the samples. CONCLUSIONS: Here, we report the high-resolution gene expression profiling of strain PE-2 during industrial-scale fermentations and the transcriptional reprograming observed under co-aggregation conditions. This dataset constitutes an important resource that can provide support for further development of this key yeast biocatalyst. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0196-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4318157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43181572015-02-06 Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production Carvalho-Netto, Osmar V Carazzolle, Marcelo F Mofatto, Luciana S Teixeira, Paulo JPL Noronha, Melline F Calderón, Luige AL Mieczkowski, Piotr A Argueso, Juan Lucas Pereira, Gonçalo AG Microb Cell Fact Research BACKGROUND: The bioethanol production system used in Brazil is based on the fermentation of sucrose from sugarcane feedstock by highly adapted strains of the yeast Saccharomyces cerevisiae. Bacterial contaminants present in the distillery environment often produce yeast-bacteria cellular co-aggregation particles that resemble yeast-yeast cell adhesion (flocculation). The formation of such particles is undesirable because it slows the fermentation kinetics and reduces the overall bioethanol yield. RESULTS: In this study, we investigated the molecular physiology of one of the main S. cerevisiae strains used in Brazilian bioethanol production, PE-2, under two contrasting conditions: typical fermentation, when most yeast cells are in suspension, and co-aggregated fermentation. The transcriptional profile of PE-2 was assessed by RNA-seq during industrial scale fed-batch fermentation. Comparative analysis between the two conditions revealed transcriptional profiles that were differentiated primarily by a deep gene repression in the co-aggregated samples. The data also indicated that Lactobacillus fermentum was likely the main bacterial species responsible for cellular co-aggregation and for the high levels of organic acids detected in the samples. CONCLUSIONS: Here, we report the high-resolution gene expression profiling of strain PE-2 during industrial-scale fermentations and the transcriptional reprograming observed under co-aggregation conditions. This dataset constitutes an important resource that can provide support for further development of this key yeast biocatalyst. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0196-6) contains supplementary material, which is available to authorized users. BioMed Central 2015-01-30 /pmc/articles/PMC4318157/ /pubmed/25633848 http://dx.doi.org/10.1186/s12934-015-0196-6 Text en © Carvalho-Netto et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Carvalho-Netto, Osmar V Carazzolle, Marcelo F Mofatto, Luciana S Teixeira, Paulo JPL Noronha, Melline F Calderón, Luige AL Mieczkowski, Piotr A Argueso, Juan Lucas Pereira, Gonçalo AG Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production |
title | Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production |
title_full | Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production |
title_fullStr | Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production |
title_full_unstemmed | Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production |
title_short | Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production |
title_sort | saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318157/ https://www.ncbi.nlm.nih.gov/pubmed/25633848 http://dx.doi.org/10.1186/s12934-015-0196-6 |
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