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Common 4977 bp deletion and novel alterations in mitochondrial DNA in Vietnamese patients with breast cancer

Mitochondrial DNA (mtDNA) has been proposed to be involved in carcinogenesis and ageing. The mtDNA 4977 bp deletion is one of the most frequently observed mtDNA mutations in human tissues and may play a role in breast cancer (BC). The aim of this study was to investigate the frequency of mtDNA 4977...

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Autores principales: Dimberg, Jan, Hong, Thai Trinh, Nguyen, Linh Tu Thi, Skarstedt, Marita, Löfgren, Sture, Matussek, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318828/
https://www.ncbi.nlm.nih.gov/pubmed/25674508
http://dx.doi.org/10.1186/s40064-015-0843-8
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author Dimberg, Jan
Hong, Thai Trinh
Nguyen, Linh Tu Thi
Skarstedt, Marita
Löfgren, Sture
Matussek, Andreas
author_facet Dimberg, Jan
Hong, Thai Trinh
Nguyen, Linh Tu Thi
Skarstedt, Marita
Löfgren, Sture
Matussek, Andreas
author_sort Dimberg, Jan
collection PubMed
description Mitochondrial DNA (mtDNA) has been proposed to be involved in carcinogenesis and ageing. The mtDNA 4977 bp deletion is one of the most frequently observed mtDNA mutations in human tissues and may play a role in breast cancer (BC). The aim of this study was to investigate the frequency of mtDNA 4977 bp deletion in BC tissue and its association with clinical factors. We determined the presence of the 4977 bp common deletion in cancer and normal paired tissue samples from 106 Vietnamese patients with BC by sequencing PCR products. The mtDNA 4977 bp deletion was significantly more frequent in normal tissue in comparison with paired cancer tissue. Moreover, the incidence of the 4977 bp deletion in BC tissue was significantly higher in patients with estrogen receptor (ER) positive as compared with ER negative BC tissue. Preliminary results showed, in cancerous tissue, a significantly higher incidence of novel deletions in the group of patients with lymph node metastasis in comparison with the patients with no lymph node metastasis. We have found 4977 bp deletion in mtDNA to be a common event in BC and with special reference to ER positive BC. In addition, the novel deletions were shown to be related to lymph node metastasis. Our finding may provide complementary information in prediction of clinical outcome including metastasis, recurrence and survival of patients with BC.
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spelling pubmed-43188282015-02-11 Common 4977 bp deletion and novel alterations in mitochondrial DNA in Vietnamese patients with breast cancer Dimberg, Jan Hong, Thai Trinh Nguyen, Linh Tu Thi Skarstedt, Marita Löfgren, Sture Matussek, Andreas Springerplus Research Mitochondrial DNA (mtDNA) has been proposed to be involved in carcinogenesis and ageing. The mtDNA 4977 bp deletion is one of the most frequently observed mtDNA mutations in human tissues and may play a role in breast cancer (BC). The aim of this study was to investigate the frequency of mtDNA 4977 bp deletion in BC tissue and its association with clinical factors. We determined the presence of the 4977 bp common deletion in cancer and normal paired tissue samples from 106 Vietnamese patients with BC by sequencing PCR products. The mtDNA 4977 bp deletion was significantly more frequent in normal tissue in comparison with paired cancer tissue. Moreover, the incidence of the 4977 bp deletion in BC tissue was significantly higher in patients with estrogen receptor (ER) positive as compared with ER negative BC tissue. Preliminary results showed, in cancerous tissue, a significantly higher incidence of novel deletions in the group of patients with lymph node metastasis in comparison with the patients with no lymph node metastasis. We have found 4977 bp deletion in mtDNA to be a common event in BC and with special reference to ER positive BC. In addition, the novel deletions were shown to be related to lymph node metastasis. Our finding may provide complementary information in prediction of clinical outcome including metastasis, recurrence and survival of patients with BC. Springer International Publishing 2015-02-03 /pmc/articles/PMC4318828/ /pubmed/25674508 http://dx.doi.org/10.1186/s40064-015-0843-8 Text en © Dimberg et al.; licensee Springer. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research
Dimberg, Jan
Hong, Thai Trinh
Nguyen, Linh Tu Thi
Skarstedt, Marita
Löfgren, Sture
Matussek, Andreas
Common 4977 bp deletion and novel alterations in mitochondrial DNA in Vietnamese patients with breast cancer
title Common 4977 bp deletion and novel alterations in mitochondrial DNA in Vietnamese patients with breast cancer
title_full Common 4977 bp deletion and novel alterations in mitochondrial DNA in Vietnamese patients with breast cancer
title_fullStr Common 4977 bp deletion and novel alterations in mitochondrial DNA in Vietnamese patients with breast cancer
title_full_unstemmed Common 4977 bp deletion and novel alterations in mitochondrial DNA in Vietnamese patients with breast cancer
title_short Common 4977 bp deletion and novel alterations in mitochondrial DNA in Vietnamese patients with breast cancer
title_sort common 4977 bp deletion and novel alterations in mitochondrial dna in vietnamese patients with breast cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318828/
https://www.ncbi.nlm.nih.gov/pubmed/25674508
http://dx.doi.org/10.1186/s40064-015-0843-8
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