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Pancreatic and Pancreatic-Like Microbial Proteases Accelerate Gut Maturation in Neonatal Rats

OBJECTIVES: Postnatal gut maturation in neonatal mammals, either at natural weaning or after precocious inducement, is coinciding with enhanced enzymes production by exocrine pancreas. Since the involvement of enzymes in gut functional maturation was overlooked, the present study aimed to investigat...

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Autores principales: Prykhodko, Olena, Pierzynowski, Stefan G., Nikpey, Elham, Arevalo Sureda, Ester, Fedkiv, Olexandr, Weström, Björn R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319746/
https://www.ncbi.nlm.nih.gov/pubmed/25658606
http://dx.doi.org/10.1371/journal.pone.0116947
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author Prykhodko, Olena
Pierzynowski, Stefan G.
Nikpey, Elham
Arevalo Sureda, Ester
Fedkiv, Olexandr
Weström, Björn R.
author_facet Prykhodko, Olena
Pierzynowski, Stefan G.
Nikpey, Elham
Arevalo Sureda, Ester
Fedkiv, Olexandr
Weström, Björn R.
author_sort Prykhodko, Olena
collection PubMed
description OBJECTIVES: Postnatal gut maturation in neonatal mammals, either at natural weaning or after precocious inducement, is coinciding with enhanced enzymes production by exocrine pancreas. Since the involvement of enzymes in gut functional maturation was overlooked, the present study aimed to investigate the role of enzymes in gut functional maturation using neonatal rats. METHODS: Suckling rats (Rattus norvegicus) were instagastrically gavaged with porcine pancreatic enzymes (Creon), microbial-derived amylase, protease, lipase and mixture thereof, while controls received α-lactalbumin or water once per day during 14–16 d of age. At 17 d of age the animals were euthanized and visceral organs were dissected, weighed and analyzed for structural and functional properties. For some of the rats, gavage with the macromolecular markers such as bovine serum albumin and bovine IgG was performed 3 hours prior to blood collection to assess the intestinal permeability. RESULTS: Gavage with the pancreatic or pancreatic-like enzymes resulted in stimulated gut growth, increased gastric acid secretion and switched intestinal disaccharidases, with decreased lactase and increased maltase and sucrase activities. The fetal-type vacuolated enterocytes were replaced by the adult-type in the distal intestine, and macromolecular transfer to the blood was declined. Enzyme exposure also promoted pancreas growth with increased amylase and trypsin production. These effects were confined to the proteases in a dose-dependent manner. CONCLUSION: Feeding exogenous enzymes, containing proteases, induced precocious gut maturation in suckling rats. This suggests that luminal exposure to proteases by oral loading or, possibly, via enhanced pancreatic secretion involves in the gut maturation of young mammals.
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spelling pubmed-43197462015-02-18 Pancreatic and Pancreatic-Like Microbial Proteases Accelerate Gut Maturation in Neonatal Rats Prykhodko, Olena Pierzynowski, Stefan G. Nikpey, Elham Arevalo Sureda, Ester Fedkiv, Olexandr Weström, Björn R. PLoS One Research Article OBJECTIVES: Postnatal gut maturation in neonatal mammals, either at natural weaning or after precocious inducement, is coinciding with enhanced enzymes production by exocrine pancreas. Since the involvement of enzymes in gut functional maturation was overlooked, the present study aimed to investigate the role of enzymes in gut functional maturation using neonatal rats. METHODS: Suckling rats (Rattus norvegicus) were instagastrically gavaged with porcine pancreatic enzymes (Creon), microbial-derived amylase, protease, lipase and mixture thereof, while controls received α-lactalbumin or water once per day during 14–16 d of age. At 17 d of age the animals were euthanized and visceral organs were dissected, weighed and analyzed for structural and functional properties. For some of the rats, gavage with the macromolecular markers such as bovine serum albumin and bovine IgG was performed 3 hours prior to blood collection to assess the intestinal permeability. RESULTS: Gavage with the pancreatic or pancreatic-like enzymes resulted in stimulated gut growth, increased gastric acid secretion and switched intestinal disaccharidases, with decreased lactase and increased maltase and sucrase activities. The fetal-type vacuolated enterocytes were replaced by the adult-type in the distal intestine, and macromolecular transfer to the blood was declined. Enzyme exposure also promoted pancreas growth with increased amylase and trypsin production. These effects were confined to the proteases in a dose-dependent manner. CONCLUSION: Feeding exogenous enzymes, containing proteases, induced precocious gut maturation in suckling rats. This suggests that luminal exposure to proteases by oral loading or, possibly, via enhanced pancreatic secretion involves in the gut maturation of young mammals. Public Library of Science 2015-02-06 /pmc/articles/PMC4319746/ /pubmed/25658606 http://dx.doi.org/10.1371/journal.pone.0116947 Text en © 2015 Prykhodko et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Prykhodko, Olena
Pierzynowski, Stefan G.
Nikpey, Elham
Arevalo Sureda, Ester
Fedkiv, Olexandr
Weström, Björn R.
Pancreatic and Pancreatic-Like Microbial Proteases Accelerate Gut Maturation in Neonatal Rats
title Pancreatic and Pancreatic-Like Microbial Proteases Accelerate Gut Maturation in Neonatal Rats
title_full Pancreatic and Pancreatic-Like Microbial Proteases Accelerate Gut Maturation in Neonatal Rats
title_fullStr Pancreatic and Pancreatic-Like Microbial Proteases Accelerate Gut Maturation in Neonatal Rats
title_full_unstemmed Pancreatic and Pancreatic-Like Microbial Proteases Accelerate Gut Maturation in Neonatal Rats
title_short Pancreatic and Pancreatic-Like Microbial Proteases Accelerate Gut Maturation in Neonatal Rats
title_sort pancreatic and pancreatic-like microbial proteases accelerate gut maturation in neonatal rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319746/
https://www.ncbi.nlm.nih.gov/pubmed/25658606
http://dx.doi.org/10.1371/journal.pone.0116947
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