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BANF1 Is Downregulated by IRF1-Regulated MicroRNA-203 in Cervical Cancer

MicroRNAs (miRNAs) play important roles in various biological processes and are closely associated with the development of cancer. In fact, aberrant expression of miRNAs has been implicated in numerous cancers. In cervical cancer, miR-203 levels are decreased, although the cause of this aberrant exp...

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Detalles Bibliográficos
Autores principales: Mao, Langyong, Zhang, Yan, Mo, Wenjuan, Yu, Yao, Lu, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319761/
https://www.ncbi.nlm.nih.gov/pubmed/25658920
http://dx.doi.org/10.1371/journal.pone.0117035
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author Mao, Langyong
Zhang, Yan
Mo, Wenjuan
Yu, Yao
Lu, Hong
author_facet Mao, Langyong
Zhang, Yan
Mo, Wenjuan
Yu, Yao
Lu, Hong
author_sort Mao, Langyong
collection PubMed
description MicroRNAs (miRNAs) play important roles in various biological processes and are closely associated with the development of cancer. In fact, aberrant expression of miRNAs has been implicated in numerous cancers. In cervical cancer, miR-203 levels are decreased, although the cause of this aberrant expression remains unclear. In this study, we investigate the molecular mechanisms regulating miR-203 gene transcription. We identify the miR-203 transcription start site by 5’ rapid amplification of cDNA ends and subsequently identify the miR-203 promoter region. Promoter analysis revealed that IRF1, a transcription factor, regulates miR-203 transcription by binding to the miR-203 promoter. We also demonstrate that miR-203 targets the 3’ untranslated region of BANF1, thus downregulating its expression, whereas miR-203 expression is driven by IRF1. MiR-203 is involved in cell cycle regulation and overexpression of miR-203 suppresses cervical cancer cell proliferation, colony formation, migration and invasion. The inhibitory effect of miR-203 on the cancer cells is partially mediated by downregulating its target, BANF1, since knockdown of BANF1 also suppresses colony formation, migration and invasion.
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spelling pubmed-43197612015-02-18 BANF1 Is Downregulated by IRF1-Regulated MicroRNA-203 in Cervical Cancer Mao, Langyong Zhang, Yan Mo, Wenjuan Yu, Yao Lu, Hong PLoS One Research Article MicroRNAs (miRNAs) play important roles in various biological processes and are closely associated with the development of cancer. In fact, aberrant expression of miRNAs has been implicated in numerous cancers. In cervical cancer, miR-203 levels are decreased, although the cause of this aberrant expression remains unclear. In this study, we investigate the molecular mechanisms regulating miR-203 gene transcription. We identify the miR-203 transcription start site by 5’ rapid amplification of cDNA ends and subsequently identify the miR-203 promoter region. Promoter analysis revealed that IRF1, a transcription factor, regulates miR-203 transcription by binding to the miR-203 promoter. We also demonstrate that miR-203 targets the 3’ untranslated region of BANF1, thus downregulating its expression, whereas miR-203 expression is driven by IRF1. MiR-203 is involved in cell cycle regulation and overexpression of miR-203 suppresses cervical cancer cell proliferation, colony formation, migration and invasion. The inhibitory effect of miR-203 on the cancer cells is partially mediated by downregulating its target, BANF1, since knockdown of BANF1 also suppresses colony formation, migration and invasion. Public Library of Science 2015-02-06 /pmc/articles/PMC4319761/ /pubmed/25658920 http://dx.doi.org/10.1371/journal.pone.0117035 Text en © 2015 Mao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mao, Langyong
Zhang, Yan
Mo, Wenjuan
Yu, Yao
Lu, Hong
BANF1 Is Downregulated by IRF1-Regulated MicroRNA-203 in Cervical Cancer
title BANF1 Is Downregulated by IRF1-Regulated MicroRNA-203 in Cervical Cancer
title_full BANF1 Is Downregulated by IRF1-Regulated MicroRNA-203 in Cervical Cancer
title_fullStr BANF1 Is Downregulated by IRF1-Regulated MicroRNA-203 in Cervical Cancer
title_full_unstemmed BANF1 Is Downregulated by IRF1-Regulated MicroRNA-203 in Cervical Cancer
title_short BANF1 Is Downregulated by IRF1-Regulated MicroRNA-203 in Cervical Cancer
title_sort banf1 is downregulated by irf1-regulated microrna-203 in cervical cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319761/
https://www.ncbi.nlm.nih.gov/pubmed/25658920
http://dx.doi.org/10.1371/journal.pone.0117035
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