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Insights into the Cellular Function of YhdE, a Nucleotide Pyrophosphatase from Escherichia coli
YhdE, a Maf-like protein in Escherichia coli, exhibits nucleotide pyrophosphatase (PPase) activity, yet its cellular function remains unknown. Here, we characterized the PPase activity of YhdE on dTTP, UTP and TTP and determined two crystal structures of YhdE, revealing ‘closed’ and ‘open’ conformat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319933/ https://www.ncbi.nlm.nih.gov/pubmed/25658941 http://dx.doi.org/10.1371/journal.pone.0117823 |
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author | Jin, Jin Wu, Ruijuan Zhu, Jia Yang, Shaoyuan Lei, Zhen Wang, Nan Singh, Vinay K. Zheng, Jimin Jia, Zongchao |
author_facet | Jin, Jin Wu, Ruijuan Zhu, Jia Yang, Shaoyuan Lei, Zhen Wang, Nan Singh, Vinay K. Zheng, Jimin Jia, Zongchao |
author_sort | Jin, Jin |
collection | PubMed |
description | YhdE, a Maf-like protein in Escherichia coli, exhibits nucleotide pyrophosphatase (PPase) activity, yet its cellular function remains unknown. Here, we characterized the PPase activity of YhdE on dTTP, UTP and TTP and determined two crystal structures of YhdE, revealing ‘closed’ and ‘open’ conformations of an adaptive active site. Our functional studies demonstrated that YhdE retards cell growth by prolonging the lag and log phases, particularly under stress conditions. Morphology studies showed that yhdE-knockout cells transformed the normal rod shape of wild-type cells to a more spherical form, and the cell wall appeared to become more flexible. In contrast, YhdE overexpression resulted in filamentous cells. This study reveals the previously unknown involvement of YhdE in cell growth inhibition under stress conditions, cell-division arrest and cell-shape maintenance, highlighting YhdE’s important role in E. coli cell-cycle checkpoints. |
format | Online Article Text |
id | pubmed-4319933 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43199332015-02-18 Insights into the Cellular Function of YhdE, a Nucleotide Pyrophosphatase from Escherichia coli Jin, Jin Wu, Ruijuan Zhu, Jia Yang, Shaoyuan Lei, Zhen Wang, Nan Singh, Vinay K. Zheng, Jimin Jia, Zongchao PLoS One Research Article YhdE, a Maf-like protein in Escherichia coli, exhibits nucleotide pyrophosphatase (PPase) activity, yet its cellular function remains unknown. Here, we characterized the PPase activity of YhdE on dTTP, UTP and TTP and determined two crystal structures of YhdE, revealing ‘closed’ and ‘open’ conformations of an adaptive active site. Our functional studies demonstrated that YhdE retards cell growth by prolonging the lag and log phases, particularly under stress conditions. Morphology studies showed that yhdE-knockout cells transformed the normal rod shape of wild-type cells to a more spherical form, and the cell wall appeared to become more flexible. In contrast, YhdE overexpression resulted in filamentous cells. This study reveals the previously unknown involvement of YhdE in cell growth inhibition under stress conditions, cell-division arrest and cell-shape maintenance, highlighting YhdE’s important role in E. coli cell-cycle checkpoints. Public Library of Science 2015-02-06 /pmc/articles/PMC4319933/ /pubmed/25658941 http://dx.doi.org/10.1371/journal.pone.0117823 Text en © 2015 Jin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jin, Jin Wu, Ruijuan Zhu, Jia Yang, Shaoyuan Lei, Zhen Wang, Nan Singh, Vinay K. Zheng, Jimin Jia, Zongchao Insights into the Cellular Function of YhdE, a Nucleotide Pyrophosphatase from Escherichia coli |
title | Insights into the Cellular Function of YhdE, a Nucleotide Pyrophosphatase from Escherichia coli
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title_full | Insights into the Cellular Function of YhdE, a Nucleotide Pyrophosphatase from Escherichia coli
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title_fullStr | Insights into the Cellular Function of YhdE, a Nucleotide Pyrophosphatase from Escherichia coli
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title_full_unstemmed | Insights into the Cellular Function of YhdE, a Nucleotide Pyrophosphatase from Escherichia coli
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title_short | Insights into the Cellular Function of YhdE, a Nucleotide Pyrophosphatase from Escherichia coli
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title_sort | insights into the cellular function of yhde, a nucleotide pyrophosphatase from escherichia coli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319933/ https://www.ncbi.nlm.nih.gov/pubmed/25658941 http://dx.doi.org/10.1371/journal.pone.0117823 |
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