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Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source

Endophytic fungi live inside plants, apparently do not cause any harm to their hosts and may play important roles in defense and growth promotion. Fungal growth is a routine practice at microbiological laboratories, and the Potato Dextrose Agar (PDA) is the most frequently used medium because it is...

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Autores principales: Sia, Eliandra de Freitas, Marcon, Joelma, Luvizotto, Danice Mazzer, Quecine, Maria Carolina, Tsui, Sarina, Pereira, José Odair, Pizzirani-Kleiner, Aline Aparecida, Azevedo, João Lúcio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320186/
https://www.ncbi.nlm.nih.gov/pubmed/25674409
http://dx.doi.org/10.1186/2193-1801-2-579
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author Sia, Eliandra de Freitas
Marcon, Joelma
Luvizotto, Danice Mazzer
Quecine, Maria Carolina
Tsui, Sarina
Pereira, José Odair
Pizzirani-Kleiner, Aline Aparecida
Azevedo, João Lúcio
author_facet Sia, Eliandra de Freitas
Marcon, Joelma
Luvizotto, Danice Mazzer
Quecine, Maria Carolina
Tsui, Sarina
Pereira, José Odair
Pizzirani-Kleiner, Aline Aparecida
Azevedo, João Lúcio
author_sort Sia, Eliandra de Freitas
collection PubMed
description Endophytic fungi live inside plants, apparently do not cause any harm to their hosts and may play important roles in defense and growth promotion. Fungal growth is a routine practice at microbiological laboratories, and the Potato Dextrose Agar (PDA) is the most frequently used medium because it is a rich source of starch. However, the production of potatoes in some regions of the world can be costly. Aiming the development of a new medium source to tropical countries, in the present study, we used leaves from the guarana (a tropical plant from the Amazon region) and the olive (which grows in subtropical and temperate regions) to isolate endophytic fungi using PDA and Manihot Dextrose Agar (MDA). Cassava (Manihot esculenta) was evaluated as a substitute starch source. For guarana, the endophytic incidence (EI) was 90% and 98% on PDA and MDA media, respectively, and 65% and 70% for olive, respectively. The fungal isolates were sequenced using the ITS- rDNA region. The fungal identification demonstrated that the isolates varied according to the host plant and media source. In the guarana plant, 13 fungal genera were found using MDA and six were found using PDA. In the olive plant, six genera were obtained using PDA and 4 were obtained using MDA. The multivariate analysis results demonstrated the highest fungal diversity from guarana when using MDA medium. Interestingly, some genera were isolated from one specific host or in one specific media, suggesting the importance of these two factors in fungal isolation specificity. Thus, this study indicated that cassava is a feasible starch source that could serve as a potential alternative medium to potato medium.
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spelling pubmed-43201862015-02-11 Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source Sia, Eliandra de Freitas Marcon, Joelma Luvizotto, Danice Mazzer Quecine, Maria Carolina Tsui, Sarina Pereira, José Odair Pizzirani-Kleiner, Aline Aparecida Azevedo, João Lúcio Springerplus Research Endophytic fungi live inside plants, apparently do not cause any harm to their hosts and may play important roles in defense and growth promotion. Fungal growth is a routine practice at microbiological laboratories, and the Potato Dextrose Agar (PDA) is the most frequently used medium because it is a rich source of starch. However, the production of potatoes in some regions of the world can be costly. Aiming the development of a new medium source to tropical countries, in the present study, we used leaves from the guarana (a tropical plant from the Amazon region) and the olive (which grows in subtropical and temperate regions) to isolate endophytic fungi using PDA and Manihot Dextrose Agar (MDA). Cassava (Manihot esculenta) was evaluated as a substitute starch source. For guarana, the endophytic incidence (EI) was 90% and 98% on PDA and MDA media, respectively, and 65% and 70% for olive, respectively. The fungal isolates were sequenced using the ITS- rDNA region. The fungal identification demonstrated that the isolates varied according to the host plant and media source. In the guarana plant, 13 fungal genera were found using MDA and six were found using PDA. In the olive plant, six genera were obtained using PDA and 4 were obtained using MDA. The multivariate analysis results demonstrated the highest fungal diversity from guarana when using MDA medium. Interestingly, some genera were isolated from one specific host or in one specific media, suggesting the importance of these two factors in fungal isolation specificity. Thus, this study indicated that cassava is a feasible starch source that could serve as a potential alternative medium to potato medium. Springer International Publishing 2013-10-30 /pmc/articles/PMC4320186/ /pubmed/25674409 http://dx.doi.org/10.1186/2193-1801-2-579 Text en © Sia et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Sia, Eliandra de Freitas
Marcon, Joelma
Luvizotto, Danice Mazzer
Quecine, Maria Carolina
Tsui, Sarina
Pereira, José Odair
Pizzirani-Kleiner, Aline Aparecida
Azevedo, João Lúcio
Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source
title Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source
title_full Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source
title_fullStr Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source
title_full_unstemmed Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source
title_short Endophytic fungi from the Amazonian plant Paullinia cupana and from Olea europaea isolated using cassava as an alternative starch media source
title_sort endophytic fungi from the amazonian plant paullinia cupana and from olea europaea isolated using cassava as an alternative starch media source
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320186/
https://www.ncbi.nlm.nih.gov/pubmed/25674409
http://dx.doi.org/10.1186/2193-1801-2-579
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