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Differential expression of Cyclin D1 in keratin-producing odontogenic cysts

Objetives: The aim of the present study was to analyze the expression levels of Cyclin D1 (CCD1), a nuclear protein that plays a crucial role in cell cycle progression, in a series of keratin-producing odontogenic cysts. Study Design: A total of 58 keratin-producing odontogenic cysts, diagnosed over...

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Autores principales: Vera-Sirera, Beatriz, Forner-Navarro, Leopoldo, Vera-Sempere, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medicina Oral S.L. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320422/
https://www.ncbi.nlm.nih.gov/pubmed/25475773
http://dx.doi.org/10.4317/medoral.20114
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author Vera-Sirera, Beatriz
Forner-Navarro, Leopoldo
Vera-Sempere, Francisco
author_facet Vera-Sirera, Beatriz
Forner-Navarro, Leopoldo
Vera-Sempere, Francisco
author_sort Vera-Sirera, Beatriz
collection PubMed
description Objetives: The aim of the present study was to analyze the expression levels of Cyclin D1 (CCD1), a nuclear protein that plays a crucial role in cell cycle progression, in a series of keratin-producing odontogenic cysts. Study Design: A total of 58 keratin-producing odontogenic cysts, diagnosed over ten years and classified according to the WHO 2005 criteria, were immunohistochemically analyzed in terms of CCD1 expression, which was quantified in the basal, suprabasal and intermediate/superficial epithelial compartments. The extent of immunostaining was measured as a proportion of total epithelial thickness. Quantified immunohistochemical data were correlated with clinicopathological features and clinical recurrence. Results: Keratin-producing odontogenic cysts were classified as 6 syndromic keratocystic odontogenic tumors (S-KCOT), 40 sporadic or non-syndromic KCOT (NS-KCOT) and 12 orthokeratinized odontogenic cysts (OOC). Immunohistochemically, CCD1 staining was evident predominantly in the parabasal region of all cystic lesions, but among-lesion differences were apparent, showing a clear expansion of parabasal compartment especially in the S-KCOT, followed to a lesser extent in the NS-KCOT, and being much more reduced in the OOC, which had the greatest average epithelial thickness. Conclusions: The differential expression of CCD1 noted in the present study suggests that dysregulation of cell cycle progression from G1 to the S phase contributes to the different aggressiveness of these lesions. However, CCD1 expression levels did not predict NS-KCOT recurrence, which is likely influenced by factors unrelated to lesion biology. Key words:Keratin-producing odontogenic cyst, keratocyst, keratocystic odontogenic tumor, nevoid basal cell carcinoma syndrome, orthokeratinized odontogenic cyst, cyclin D1, immunohistochemistry.
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spelling pubmed-43204222015-02-13 Differential expression of Cyclin D1 in keratin-producing odontogenic cysts Vera-Sirera, Beatriz Forner-Navarro, Leopoldo Vera-Sempere, Francisco Med Oral Patol Oral Cir Bucal Research Objetives: The aim of the present study was to analyze the expression levels of Cyclin D1 (CCD1), a nuclear protein that plays a crucial role in cell cycle progression, in a series of keratin-producing odontogenic cysts. Study Design: A total of 58 keratin-producing odontogenic cysts, diagnosed over ten years and classified according to the WHO 2005 criteria, were immunohistochemically analyzed in terms of CCD1 expression, which was quantified in the basal, suprabasal and intermediate/superficial epithelial compartments. The extent of immunostaining was measured as a proportion of total epithelial thickness. Quantified immunohistochemical data were correlated with clinicopathological features and clinical recurrence. Results: Keratin-producing odontogenic cysts were classified as 6 syndromic keratocystic odontogenic tumors (S-KCOT), 40 sporadic or non-syndromic KCOT (NS-KCOT) and 12 orthokeratinized odontogenic cysts (OOC). Immunohistochemically, CCD1 staining was evident predominantly in the parabasal region of all cystic lesions, but among-lesion differences were apparent, showing a clear expansion of parabasal compartment especially in the S-KCOT, followed to a lesser extent in the NS-KCOT, and being much more reduced in the OOC, which had the greatest average epithelial thickness. Conclusions: The differential expression of CCD1 noted in the present study suggests that dysregulation of cell cycle progression from G1 to the S phase contributes to the different aggressiveness of these lesions. However, CCD1 expression levels did not predict NS-KCOT recurrence, which is likely influenced by factors unrelated to lesion biology. Key words:Keratin-producing odontogenic cyst, keratocyst, keratocystic odontogenic tumor, nevoid basal cell carcinoma syndrome, orthokeratinized odontogenic cyst, cyclin D1, immunohistochemistry. Medicina Oral S.L. 2015-01 2015-01-05 /pmc/articles/PMC4320422/ /pubmed/25475773 http://dx.doi.org/10.4317/medoral.20114 Text en Copyright: © 2015 Medicina Oral S.L. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Vera-Sirera, Beatriz
Forner-Navarro, Leopoldo
Vera-Sempere, Francisco
Differential expression of Cyclin D1 in keratin-producing odontogenic cysts
title Differential expression of Cyclin D1 in keratin-producing odontogenic cysts
title_full Differential expression of Cyclin D1 in keratin-producing odontogenic cysts
title_fullStr Differential expression of Cyclin D1 in keratin-producing odontogenic cysts
title_full_unstemmed Differential expression of Cyclin D1 in keratin-producing odontogenic cysts
title_short Differential expression of Cyclin D1 in keratin-producing odontogenic cysts
title_sort differential expression of cyclin d1 in keratin-producing odontogenic cysts
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320422/
https://www.ncbi.nlm.nih.gov/pubmed/25475773
http://dx.doi.org/10.4317/medoral.20114
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