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Evaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells

BACKGROUND: Although profiling of RNA in single cells has broadened our understanding of development, cancer biology and mechanisms of disease dissemination, it requires the development of reliable and flexible methods. Here we demonstrate that the EpiStem RNA-Amp™ methodology reproducibly generates...

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Detalles Bibliográficos
Autores principales: Rothwell, Dominic G, Li, Yaoyong, Ayub, Mahmood, Tate, Catriona, Newton, Gillian, Hey, Yvonne, Carter, Louise, Faulkner, Suzanne, Moro, Massimo, Pepper, Stuart, Miller, Crispin, Blackhall, Fiona, Bertolini, Giulia, Roz, Luca, Dive, Caroline, Brady, Ged
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320548/
https://www.ncbi.nlm.nih.gov/pubmed/25519510
http://dx.doi.org/10.1186/1471-2164-15-1129