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Facile synthesis of fluorescent Au/Ce nanoclusters for high-sensitive bioimaging

BACKGROUND: Tumor-target fluorescence bioimaging is an important means of early diagnosis, metal nanoclusters have been used as an excellent fluorescent probe for marking tumor cells due to their targeted absorption. We have developed a new strategy for facile synthesis of Au/Ce nanoclusters (NCs) b...

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Autores principales: Ge, Wei, Zhang, Yuanyuan, Ye, Jing, Chen, Donghua, Rehman, Fawad Ur, Li, Qiwei, Chen, Yun, Jiang, Hui, Wang, Xuemei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320607/
https://www.ncbi.nlm.nih.gov/pubmed/25643754
http://dx.doi.org/10.1186/s12951-015-0071-y
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author Ge, Wei
Zhang, Yuanyuan
Ye, Jing
Chen, Donghua
Rehman, Fawad Ur
Li, Qiwei
Chen, Yun
Jiang, Hui
Wang, Xuemei
author_facet Ge, Wei
Zhang, Yuanyuan
Ye, Jing
Chen, Donghua
Rehman, Fawad Ur
Li, Qiwei
Chen, Yun
Jiang, Hui
Wang, Xuemei
author_sort Ge, Wei
collection PubMed
description BACKGROUND: Tumor-target fluorescence bioimaging is an important means of early diagnosis, metal nanoclusters have been used as an excellent fluorescent probe for marking tumor cells due to their targeted absorption. We have developed a new strategy for facile synthesis of Au/Ce nanoclusters (NCs) by doping trivalent cerium ion into seed crystal growth process of gold. Au/Ce NCs have bright fluorescence which could be used as fluorescent probe for bioimaging. RESULTS: In this study, we synthesized fluorescent Au/Ce NCs through two-step hydrothermal reaction. The concentration range of 25–350 μM, Au/Ce NCs have no obvious cell cytotoxicity effect on HeLa, HepG2 and L02 cells. Furthermore, normal cells (L02) have no obvious absorption of Au/Ce NCs. Characterization of synthesized Au/Ce NCs was done by using TEM, EDS and XPS. Then these prepared Au/Ce NCs were applied for in vitro/in vivo tumor-target bioimaging due to its prolonged fluorescence lifetime and bright luminescence properties. CONCLUSIONS: The glutathione stabilized Au/Ce NCs synthesized through hydrothermal reaction possess stable and bright fluorescence that can be readily utilized for high sensitive fluorescence probe. Our results suggest that Au/Ce NCs are useful candidate for in vitro/in vivo tumor bioimaging in potential clinical application.
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spelling pubmed-43206072015-02-08 Facile synthesis of fluorescent Au/Ce nanoclusters for high-sensitive bioimaging Ge, Wei Zhang, Yuanyuan Ye, Jing Chen, Donghua Rehman, Fawad Ur Li, Qiwei Chen, Yun Jiang, Hui Wang, Xuemei J Nanobiotechnology Research BACKGROUND: Tumor-target fluorescence bioimaging is an important means of early diagnosis, metal nanoclusters have been used as an excellent fluorescent probe for marking tumor cells due to their targeted absorption. We have developed a new strategy for facile synthesis of Au/Ce nanoclusters (NCs) by doping trivalent cerium ion into seed crystal growth process of gold. Au/Ce NCs have bright fluorescence which could be used as fluorescent probe for bioimaging. RESULTS: In this study, we synthesized fluorescent Au/Ce NCs through two-step hydrothermal reaction. The concentration range of 25–350 μM, Au/Ce NCs have no obvious cell cytotoxicity effect on HeLa, HepG2 and L02 cells. Furthermore, normal cells (L02) have no obvious absorption of Au/Ce NCs. Characterization of synthesized Au/Ce NCs was done by using TEM, EDS and XPS. Then these prepared Au/Ce NCs were applied for in vitro/in vivo tumor-target bioimaging due to its prolonged fluorescence lifetime and bright luminescence properties. CONCLUSIONS: The glutathione stabilized Au/Ce NCs synthesized through hydrothermal reaction possess stable and bright fluorescence that can be readily utilized for high sensitive fluorescence probe. Our results suggest that Au/Ce NCs are useful candidate for in vitro/in vivo tumor bioimaging in potential clinical application. BioMed Central 2015-02-03 /pmc/articles/PMC4320607/ /pubmed/25643754 http://dx.doi.org/10.1186/s12951-015-0071-y Text en © Ge et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ge, Wei
Zhang, Yuanyuan
Ye, Jing
Chen, Donghua
Rehman, Fawad Ur
Li, Qiwei
Chen, Yun
Jiang, Hui
Wang, Xuemei
Facile synthesis of fluorescent Au/Ce nanoclusters for high-sensitive bioimaging
title Facile synthesis of fluorescent Au/Ce nanoclusters for high-sensitive bioimaging
title_full Facile synthesis of fluorescent Au/Ce nanoclusters for high-sensitive bioimaging
title_fullStr Facile synthesis of fluorescent Au/Ce nanoclusters for high-sensitive bioimaging
title_full_unstemmed Facile synthesis of fluorescent Au/Ce nanoclusters for high-sensitive bioimaging
title_short Facile synthesis of fluorescent Au/Ce nanoclusters for high-sensitive bioimaging
title_sort facile synthesis of fluorescent au/ce nanoclusters for high-sensitive bioimaging
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320607/
https://www.ncbi.nlm.nih.gov/pubmed/25643754
http://dx.doi.org/10.1186/s12951-015-0071-y
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