Cargando…
Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures
The plant phenol trans-resveratrol, which is mainly found in grape, displays a wide range of biological effects. A cell suspension culture was developed from calli of grape leaves of Vitis vinifera cv. Negramaro in order to study the bioproduction of resveratrol. The effects of a number of secondary...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320690/ https://www.ncbi.nlm.nih.gov/pubmed/25674504 http://dx.doi.org/10.1186/s40064-015-0831-z |
_version_ | 1782356162597879808 |
---|---|
author | Taurino, Marco Ingrosso, Ilaria D’amico, Leone De Domenico, Stefania Nicoletti, Isabella Corradini, Danilo Santino, Angelo Giovinazzo, Giovanna |
author_facet | Taurino, Marco Ingrosso, Ilaria D’amico, Leone De Domenico, Stefania Nicoletti, Isabella Corradini, Danilo Santino, Angelo Giovinazzo, Giovanna |
author_sort | Taurino, Marco |
collection | PubMed |
description | The plant phenol trans-resveratrol, which is mainly found in grape, displays a wide range of biological effects. A cell suspension culture was developed from calli of grape leaves of Vitis vinifera cv. Negramaro in order to study the bioproduction of resveratrol. The effects of a number of secondary plant metabolism elicitors, namely chitosan, methyl jasmonate, jasmonic acid, coronatine, and 12-oxo-phytodienoic acid, were tested on this cell suspension culture. The identification and quantification of stilbenes was achieved with high performance liquid chromatography, with both spectrophotometric and mass spectrometric detection. Of the tested elicitors, methyl jasmonate was the most effective in inducing the biosynthesis of approximately 4 mg g(−1) dry weight (about 60 mg L(−1)) of resveratrol. Conversely, 12-oxo-phytodienoic acid, jasmonic acid, and coronatine were able to trigger the synthesis of approximately 20 mg g(−1) dry weight (200–210 mg L(−1)) of viniferins. Taken together, our results show for the first time different modulatory effects of closely-related jasmonates on stilbene biosynthesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-015-0831-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4320690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-43206902015-02-11 Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures Taurino, Marco Ingrosso, Ilaria D’amico, Leone De Domenico, Stefania Nicoletti, Isabella Corradini, Danilo Santino, Angelo Giovinazzo, Giovanna Springerplus Research The plant phenol trans-resveratrol, which is mainly found in grape, displays a wide range of biological effects. A cell suspension culture was developed from calli of grape leaves of Vitis vinifera cv. Negramaro in order to study the bioproduction of resveratrol. The effects of a number of secondary plant metabolism elicitors, namely chitosan, methyl jasmonate, jasmonic acid, coronatine, and 12-oxo-phytodienoic acid, were tested on this cell suspension culture. The identification and quantification of stilbenes was achieved with high performance liquid chromatography, with both spectrophotometric and mass spectrometric detection. Of the tested elicitors, methyl jasmonate was the most effective in inducing the biosynthesis of approximately 4 mg g(−1) dry weight (about 60 mg L(−1)) of resveratrol. Conversely, 12-oxo-phytodienoic acid, jasmonic acid, and coronatine were able to trigger the synthesis of approximately 20 mg g(−1) dry weight (200–210 mg L(−1)) of viniferins. Taken together, our results show for the first time different modulatory effects of closely-related jasmonates on stilbene biosynthesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-015-0831-z) contains supplementary material, which is available to authorized users. Springer International Publishing 2015-02-01 /pmc/articles/PMC4320690/ /pubmed/25674504 http://dx.doi.org/10.1186/s40064-015-0831-z Text en © Taurino et al.; licensee Springer. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Research Taurino, Marco Ingrosso, Ilaria D’amico, Leone De Domenico, Stefania Nicoletti, Isabella Corradini, Danilo Santino, Angelo Giovinazzo, Giovanna Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures |
title | Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures |
title_full | Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures |
title_fullStr | Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures |
title_full_unstemmed | Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures |
title_short | Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures |
title_sort | jasmonates elicit different sets of stilbenes in vitis vinifera cv. negramaro cell cultures |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320690/ https://www.ncbi.nlm.nih.gov/pubmed/25674504 http://dx.doi.org/10.1186/s40064-015-0831-z |
work_keys_str_mv | AT taurinomarco jasmonateselicitdifferentsetsofstilbenesinvitisviniferacvnegramarocellcultures AT ingrossoilaria jasmonateselicitdifferentsetsofstilbenesinvitisviniferacvnegramarocellcultures AT damicoleone jasmonateselicitdifferentsetsofstilbenesinvitisviniferacvnegramarocellcultures AT dedomenicostefania jasmonateselicitdifferentsetsofstilbenesinvitisviniferacvnegramarocellcultures AT nicolettiisabella jasmonateselicitdifferentsetsofstilbenesinvitisviniferacvnegramarocellcultures AT corradinidanilo jasmonateselicitdifferentsetsofstilbenesinvitisviniferacvnegramarocellcultures AT santinoangelo jasmonateselicitdifferentsetsofstilbenesinvitisviniferacvnegramarocellcultures AT giovinazzogiovanna jasmonateselicitdifferentsetsofstilbenesinvitisviniferacvnegramarocellcultures |