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Discordance of DNA Methylation Variance Between two Accessible Human Tissues

Population epigenetic studies have been seeking to identify differences in DNA methylation between specific exposures, demographic factors, or diseases in accessible tissues, but relatively little is known about how inter-individual variability differs between these tissues. This study presents an a...

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Autores principales: Jiang, Ruiwei, Jones, Meaghan J., Chen, Edith, Neumann, Sarah M., Fraser, Hunter B., Miller, Gregory E., Kobor, Michael S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321176/
https://www.ncbi.nlm.nih.gov/pubmed/25660083
http://dx.doi.org/10.1038/srep08257
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author Jiang, Ruiwei
Jones, Meaghan J.
Chen, Edith
Neumann, Sarah M.
Fraser, Hunter B.
Miller, Gregory E.
Kobor, Michael S.
author_facet Jiang, Ruiwei
Jones, Meaghan J.
Chen, Edith
Neumann, Sarah M.
Fraser, Hunter B.
Miller, Gregory E.
Kobor, Michael S.
author_sort Jiang, Ruiwei
collection PubMed
description Population epigenetic studies have been seeking to identify differences in DNA methylation between specific exposures, demographic factors, or diseases in accessible tissues, but relatively little is known about how inter-individual variability differs between these tissues. This study presents an analysis of DNA methylation differences between matched peripheral blood mononuclear cells (PMBCs) and buccal epithelial cells (BECs), the two most accessible tissues for population studies, in 998 promoter-located CpG sites. Specifically we compared probe-wise DNA methylation variance, and how this variance related to demographic factors across the two tissues. PBMCs had overall higher DNA methylation than BECs, and the two tissues tended to differ most at genomic regions of low CpG density. Furthermore, although both tissues showed appreciable probe-wise variability, the specific regions and magnitude of variability differed strongly between tissues. Lastly, through exploratory association analysis, we found indication of differential association of BEC and PBMC with demographic variables. The work presented here offers insight into variability of DNA methylation between individuals and across tissues and helps guide decisions on the suitability of buccal epithelial or peripheral mononuclear cells for the biological questions explored by epigenetic studies in human populations.
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spelling pubmed-43211762015-02-12 Discordance of DNA Methylation Variance Between two Accessible Human Tissues Jiang, Ruiwei Jones, Meaghan J. Chen, Edith Neumann, Sarah M. Fraser, Hunter B. Miller, Gregory E. Kobor, Michael S. Sci Rep Article Population epigenetic studies have been seeking to identify differences in DNA methylation between specific exposures, demographic factors, or diseases in accessible tissues, but relatively little is known about how inter-individual variability differs between these tissues. This study presents an analysis of DNA methylation differences between matched peripheral blood mononuclear cells (PMBCs) and buccal epithelial cells (BECs), the two most accessible tissues for population studies, in 998 promoter-located CpG sites. Specifically we compared probe-wise DNA methylation variance, and how this variance related to demographic factors across the two tissues. PBMCs had overall higher DNA methylation than BECs, and the two tissues tended to differ most at genomic regions of low CpG density. Furthermore, although both tissues showed appreciable probe-wise variability, the specific regions and magnitude of variability differed strongly between tissues. Lastly, through exploratory association analysis, we found indication of differential association of BEC and PBMC with demographic variables. The work presented here offers insight into variability of DNA methylation between individuals and across tissues and helps guide decisions on the suitability of buccal epithelial or peripheral mononuclear cells for the biological questions explored by epigenetic studies in human populations. Nature Publishing Group 2015-02-09 /pmc/articles/PMC4321176/ /pubmed/25660083 http://dx.doi.org/10.1038/srep08257 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jiang, Ruiwei
Jones, Meaghan J.
Chen, Edith
Neumann, Sarah M.
Fraser, Hunter B.
Miller, Gregory E.
Kobor, Michael S.
Discordance of DNA Methylation Variance Between two Accessible Human Tissues
title Discordance of DNA Methylation Variance Between two Accessible Human Tissues
title_full Discordance of DNA Methylation Variance Between two Accessible Human Tissues
title_fullStr Discordance of DNA Methylation Variance Between two Accessible Human Tissues
title_full_unstemmed Discordance of DNA Methylation Variance Between two Accessible Human Tissues
title_short Discordance of DNA Methylation Variance Between two Accessible Human Tissues
title_sort discordance of dna methylation variance between two accessible human tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321176/
https://www.ncbi.nlm.nih.gov/pubmed/25660083
http://dx.doi.org/10.1038/srep08257
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