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RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA

Ribonucleic acid-mediated transcriptional gene silencing (known as RNA-directed DNA methylation, or RdDM, in Arabidopsis thaliana) is important for influencing gene expression and the inhibition of transposons by the deposition of repressive chromatin marks such as histone modifications and DNA meth...

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Autores principales: Böhmdorfer, Gudrun, Rowley, M Jordan, Kuciński, Jan, Zhu, Yongyou, Amies, Ivan, Wierzbicki, Andrzej T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321213/
https://www.ncbi.nlm.nih.gov/pubmed/24862207
http://dx.doi.org/10.1111/tpj.12563
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author Böhmdorfer, Gudrun
Rowley, M Jordan
Kuciński, Jan
Zhu, Yongyou
Amies, Ivan
Wierzbicki, Andrzej T
author_facet Böhmdorfer, Gudrun
Rowley, M Jordan
Kuciński, Jan
Zhu, Yongyou
Amies, Ivan
Wierzbicki, Andrzej T
author_sort Böhmdorfer, Gudrun
collection PubMed
description Ribonucleic acid-mediated transcriptional gene silencing (known as RNA-directed DNA methylation, or RdDM, in Arabidopsis thaliana) is important for influencing gene expression and the inhibition of transposons by the deposition of repressive chromatin marks such as histone modifications and DNA methylation. A key event in de novo methylation of DNA by RdDM is the production of long non-coding RNA (lncRNA) by RNA polymerase V (Pol V). Little is known about the events that connect Pol V transcription to the establishment of repressive chromatin modifications. Using RNA immunoprecipitation, we elucidated the order of events downstream of lncRNA production and discovered interdependency between lncRNA-associated proteins. We found that the effector protein ARGONAUTE4 (AGO4) binds lncRNA independent of the RNA-binding protein INVOLVED IN DE NOVO2 (IDN2). In contrast, IDN2 binds lncRNA in an AGO4-dependent manner. We further found that the de novo DNA methyltransferase DOMAINS REARRANGED METHYLTRANSFERASE2 (DRM2) also associates with lncRNA produced by Pol V and that this event depends on AGO4 and IDN2. We propose a model where the silencing proteins AGO4, IDN2 and DRM2 bind to lncRNA in a stepwise manner, resulting in DNA methylation of RdDM target loci.
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spelling pubmed-43212132015-02-13 RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA Böhmdorfer, Gudrun Rowley, M Jordan Kuciński, Jan Zhu, Yongyou Amies, Ivan Wierzbicki, Andrzej T Plant J Featured Article Ribonucleic acid-mediated transcriptional gene silencing (known as RNA-directed DNA methylation, or RdDM, in Arabidopsis thaliana) is important for influencing gene expression and the inhibition of transposons by the deposition of repressive chromatin marks such as histone modifications and DNA methylation. A key event in de novo methylation of DNA by RdDM is the production of long non-coding RNA (lncRNA) by RNA polymerase V (Pol V). Little is known about the events that connect Pol V transcription to the establishment of repressive chromatin modifications. Using RNA immunoprecipitation, we elucidated the order of events downstream of lncRNA production and discovered interdependency between lncRNA-associated proteins. We found that the effector protein ARGONAUTE4 (AGO4) binds lncRNA independent of the RNA-binding protein INVOLVED IN DE NOVO2 (IDN2). In contrast, IDN2 binds lncRNA in an AGO4-dependent manner. We further found that the de novo DNA methyltransferase DOMAINS REARRANGED METHYLTRANSFERASE2 (DRM2) also associates with lncRNA produced by Pol V and that this event depends on AGO4 and IDN2. We propose a model where the silencing proteins AGO4, IDN2 and DRM2 bind to lncRNA in a stepwise manner, resulting in DNA methylation of RdDM target loci. BlackWell Publishing Ltd 2014-07 2014-06-23 /pmc/articles/PMC4321213/ /pubmed/24862207 http://dx.doi.org/10.1111/tpj.12563 Text en © 2014 The Authors The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Featured Article
Böhmdorfer, Gudrun
Rowley, M Jordan
Kuciński, Jan
Zhu, Yongyou
Amies, Ivan
Wierzbicki, Andrzej T
RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA
title RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA
title_full RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA
title_fullStr RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA
title_full_unstemmed RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA
title_short RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA
title_sort rna-directed dna methylation requires stepwise binding of silencing factors to long non-coding rna
topic Featured Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321213/
https://www.ncbi.nlm.nih.gov/pubmed/24862207
http://dx.doi.org/10.1111/tpj.12563
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