Endogenous biotin-binding proteins: an overlooked factor causing false positives in streptavidin-based protein detection

Biotinylation is widely used in DNA, RNA and protein probing assays as this molecule has generally no impact on the biological activity of its substrate. During the streptavidin-based detection of glycoproteins in Lactobacillus rhamnosus GG with biotinylated lectin probes, a strong positive band of...

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Detalles Bibliográficos
Autores principales: Tytgat, Hanne L P, Schoofs, Geert, Driesen, Michèle, Proost, Paul, Van Damme, Els J M, Vanderleyden, Jos, Lebeer, Sarah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321382/
https://www.ncbi.nlm.nih.gov/pubmed/25211245
http://dx.doi.org/10.1111/1751-7915.12150

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