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Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae

The protein Yju3p is the orthologue of monoglyceride lipases in the yeast Saccharomyces cerevisiae. A soluble variant of this lipase termed s-Yju3p (38.3 kDa) was generated and purified to homogeneity by affinity and size-exclusion chromatography. s-Yju3p was crystallized in a vapour-diffusion setup...

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Detalles Bibliográficos
Autores principales:	Rengachari, Srinivasan, Aschauer, Philipp, Sturm, Christian, Oberer, Monika
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	International Union of Crystallography 2015
Materias:	Research Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321484/ https://www.ncbi.nlm.nih.gov/pubmed/25664804 http://dx.doi.org/10.1107/S2053230X15001557

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