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Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study

BACKGROUND: Traditional Uighur medicine shares an origin with Greco-Arab medicine. It describes the health of a human body as the dynamic homeostasis of four normal Hilits (humours), known as Kan, Phlegm, Safra, and Savda. An abnormal change in one Hilit may cause imbalance among the Hilits, leading...

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Autores principales: Upur, Halmurat, Chen, Yin, Kamilijiang, Mayila, Deng, Wanli, Sulaiman, Xierzhatijiang, Aizezi, Renaguli, Wu, Xiao, Tulake, Wuniqiemu, Abudula, Abulizi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321703/
https://www.ncbi.nlm.nih.gov/pubmed/25652121
http://dx.doi.org/10.1186/s12906-015-0526-6
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author Upur, Halmurat
Chen, Yin
Kamilijiang, Mayila
Deng, Wanli
Sulaiman, Xierzhatijiang
Aizezi, Renaguli
Wu, Xiao
Tulake, Wuniqiemu
Abudula, Abulizi
author_facet Upur, Halmurat
Chen, Yin
Kamilijiang, Mayila
Deng, Wanli
Sulaiman, Xierzhatijiang
Aizezi, Renaguli
Wu, Xiao
Tulake, Wuniqiemu
Abudula, Abulizi
author_sort Upur, Halmurat
collection PubMed
description BACKGROUND: Traditional Uighur medicine shares an origin with Greco-Arab medicine. It describes the health of a human body as the dynamic homeostasis of four normal Hilits (humours), known as Kan, Phlegm, Safra, and Savda. An abnormal change in one Hilit may cause imbalance among the Hilits, leading to the development of a syndrome. Abnormal Savda is a major syndrome of complex diseases that are associated with common biological changes during disease development. Here, we studied the protein expression profile common to tumour patients with Abnormal Savda to elucidate the biological basis of this syndrome and identify potential biomarkers associated with Abnormal Savda. METHODS: Patients with malignant tumours were classified by the diagnosis of Uighur medicine into two groups: Abnormal Savda type tumour (ASt) and non-Abnormal Savda type tumour (nASt), which includes other syndromes. The profile of proteins that were differentially expressed in ASt compared with nASt and normal controls (NC) was analysed by iTRAQ proteomics and evaluated by bioinformatics using MetaCore™ software and an online database. The expression of candidate proteins was verified in all plasma samples by enzyme-linked immunosorbent assay (ELISA). RESULTS: We identified 31 plasma proteins that were differentially expressed in ASt compared with nASt, of which only 10 showed quantitatively different expression between ASt and NC. Bioinformatics analysis indicated that most of these proteins are known biomarkers for neoplasms of the stomach, breast, and lung. ELISA detection showed significant upregulation of plasma SAA1 and SPP24 and downregulation of PIGR and FASN in ASt compared with nASt and NC (p < 0.05). CONCLUSIONS: Abnormal Savda may be causally associated with changes in the whole regulation network of protein expression during carcinogenesis. The expression of potential biomarkers might be used to distinguish Abnormal Savda from other syndromes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-015-0526-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-43217032015-02-10 Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Upur, Halmurat Chen, Yin Kamilijiang, Mayila Deng, Wanli Sulaiman, Xierzhatijiang Aizezi, Renaguli Wu, Xiao Tulake, Wuniqiemu Abudula, Abulizi BMC Complement Altern Med Research Article BACKGROUND: Traditional Uighur medicine shares an origin with Greco-Arab medicine. It describes the health of a human body as the dynamic homeostasis of four normal Hilits (humours), known as Kan, Phlegm, Safra, and Savda. An abnormal change in one Hilit may cause imbalance among the Hilits, leading to the development of a syndrome. Abnormal Savda is a major syndrome of complex diseases that are associated with common biological changes during disease development. Here, we studied the protein expression profile common to tumour patients with Abnormal Savda to elucidate the biological basis of this syndrome and identify potential biomarkers associated with Abnormal Savda. METHODS: Patients with malignant tumours were classified by the diagnosis of Uighur medicine into two groups: Abnormal Savda type tumour (ASt) and non-Abnormal Savda type tumour (nASt), which includes other syndromes. The profile of proteins that were differentially expressed in ASt compared with nASt and normal controls (NC) was analysed by iTRAQ proteomics and evaluated by bioinformatics using MetaCore™ software and an online database. The expression of candidate proteins was verified in all plasma samples by enzyme-linked immunosorbent assay (ELISA). RESULTS: We identified 31 plasma proteins that were differentially expressed in ASt compared with nASt, of which only 10 showed quantitatively different expression between ASt and NC. Bioinformatics analysis indicated that most of these proteins are known biomarkers for neoplasms of the stomach, breast, and lung. ELISA detection showed significant upregulation of plasma SAA1 and SPP24 and downregulation of PIGR and FASN in ASt compared with nASt and NC (p < 0.05). CONCLUSIONS: Abnormal Savda may be causally associated with changes in the whole regulation network of protein expression during carcinogenesis. The expression of potential biomarkers might be used to distinguish Abnormal Savda from other syndromes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-015-0526-6) contains supplementary material, which is available to authorized users. BioMed Central 2015-02-05 /pmc/articles/PMC4321703/ /pubmed/25652121 http://dx.doi.org/10.1186/s12906-015-0526-6 Text en © Upur et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Upur, Halmurat
Chen, Yin
Kamilijiang, Mayila
Deng, Wanli
Sulaiman, Xierzhatijiang
Aizezi, Renaguli
Wu, Xiao
Tulake, Wuniqiemu
Abudula, Abulizi
Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study
title Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study
title_full Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study
title_fullStr Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study
title_full_unstemmed Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study
title_short Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study
title_sort identification of plasma protein markers common to patients with malignant tumour and abnormal savda in uighur medicine: a prospective clinical study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321703/
https://www.ncbi.nlm.nih.gov/pubmed/25652121
http://dx.doi.org/10.1186/s12906-015-0526-6
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