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Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers
Background: The aim of this research was to study the distribution and changes of glycoconjugates particularly their terminal sugars by using lectin histochemistry during mouse spinal cord development. Methods: Formalin-fixed sections of mouse embryo (10-16 fetal days) were processed for lectin hist...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pasteur Institute of Iran
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322235/ https://www.ncbi.nlm.nih.gov/pubmed/25605492 http://dx.doi.org/10.6091/ibj.1298.2015 |
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author | Vojoudi, Elham Ebrahimi, Vahid Ebrahimzadeh-Bideskan, Alireza Fazel, Alireza |
author_facet | Vojoudi, Elham Ebrahimi, Vahid Ebrahimzadeh-Bideskan, Alireza Fazel, Alireza |
author_sort | Vojoudi, Elham |
collection | PubMed |
description | Background: The aim of this research was to study the distribution and changes of glycoconjugates particularly their terminal sugars by using lectin histochemistry during mouse spinal cord development. Methods: Formalin-fixed sections of mouse embryo (10-16 fetal days) were processed for lectin histochemical method. In this study, two groups of horseradish peroxidase-labeled specific lectins were used: N-acetylgalactosamine, including Dolichos biflorus, Wisteria floribunda agglutinin (WFA), Vicia villosa, Glycine max as well as focuse-binding lectins, including tetragonolobus, Ulex europaeus, and Orange peel fungus (OFA). All sections were counterstained with alcian blue (pH 2.5). Results: Our results showed that only WFA and OFA reacted strongly with the floor plate cells from early to late embryonic period of developing spinal cord. The strongest reactions were related to the 14, 15, and 16 days of tissue sections incubated with OFA and WFA lectins. Conclusion: The present study demonstrated that cellular and molecular differentiation of the spinal cord organizers is a wholly regulated process, and α-L-fucose, α-D-GalNAc, and α/β-D-GalNAc terminal sugars play a significant role during the prenatal spinal cord development. |
format | Online Article Text |
id | pubmed-4322235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Pasteur Institute of Iran |
record_format | MEDLINE/PubMed |
spelling | pubmed-43222352015-02-17 Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers Vojoudi, Elham Ebrahimi, Vahid Ebrahimzadeh-Bideskan, Alireza Fazel, Alireza Iran Biomed J Short Report Background: The aim of this research was to study the distribution and changes of glycoconjugates particularly their terminal sugars by using lectin histochemistry during mouse spinal cord development. Methods: Formalin-fixed sections of mouse embryo (10-16 fetal days) were processed for lectin histochemical method. In this study, two groups of horseradish peroxidase-labeled specific lectins were used: N-acetylgalactosamine, including Dolichos biflorus, Wisteria floribunda agglutinin (WFA), Vicia villosa, Glycine max as well as focuse-binding lectins, including tetragonolobus, Ulex europaeus, and Orange peel fungus (OFA). All sections were counterstained with alcian blue (pH 2.5). Results: Our results showed that only WFA and OFA reacted strongly with the floor plate cells from early to late embryonic period of developing spinal cord. The strongest reactions were related to the 14, 15, and 16 days of tissue sections incubated with OFA and WFA lectins. Conclusion: The present study demonstrated that cellular and molecular differentiation of the spinal cord organizers is a wholly regulated process, and α-L-fucose, α-D-GalNAc, and α/β-D-GalNAc terminal sugars play a significant role during the prenatal spinal cord development. Pasteur Institute of Iran 2015-01 /pmc/articles/PMC4322235/ /pubmed/25605492 http://dx.doi.org/10.6091/ibj.1298.2015 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Vojoudi, Elham Ebrahimi, Vahid Ebrahimzadeh-Bideskan, Alireza Fazel, Alireza Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers |
title | Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers |
title_full | Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers |
title_fullStr | Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers |
title_full_unstemmed | Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers |
title_short | Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers |
title_sort | glycoconjugates distribution during developing mouse spinal cord motor organizers |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322235/ https://www.ncbi.nlm.nih.gov/pubmed/25605492 http://dx.doi.org/10.6091/ibj.1298.2015 |
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