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The regulatory network of ThbZIP1 in response to abscisic acid treatment

Previously, a bZIP transcription factor from Tamarix hispida, ThbZIP1, was characterized: plants overexpressing ThbZIP1 displayed improved salt stress tolerance but were sensitive to abscisic acid (ABA). In the current study, we further characterized the regulatory network of ThbZIP1 and the mechani...

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Autores principales: Ji, Xiaoyu, Liu, Guifeng, Liu, Yujia, Nie, Xianguang, Zheng, Lei, Wang, Yucheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322638/
https://www.ncbi.nlm.nih.gov/pubmed/25713576
http://dx.doi.org/10.3389/fpls.2015.00025
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author Ji, Xiaoyu
Liu, Guifeng
Liu, Yujia
Nie, Xianguang
Zheng, Lei
Wang, Yucheng
author_facet Ji, Xiaoyu
Liu, Guifeng
Liu, Yujia
Nie, Xianguang
Zheng, Lei
Wang, Yucheng
author_sort Ji, Xiaoyu
collection PubMed
description Previously, a bZIP transcription factor from Tamarix hispida, ThbZIP1, was characterized: plants overexpressing ThbZIP1 displayed improved salt stress tolerance but were sensitive to abscisic acid (ABA). In the current study, we further characterized the regulatory network of ThbZIP1 and the mechanism of ABA sensitivity mediated by ThbZIP1. An ABF transcription factor from T. hispida, ThABF1, directly regulates the expression of ThbZIP1. Microarray analysis identified 1662 and 1609 genes that were respectively significantly upregulated or downregulated by ThbZIP1 when exposed to ABA. Gene ontology (GO) analysis showed that the processes including “response to stimulus,” “catalytic activity,” “binding function,” and “metabolic process” were highly altered in ThbZIP1 expressing plants exposed to ABA. The gene expression in ThbZIP1 transformed plants were compared between exposed to ABA and salt on the genome scale. Genes differentially regulated by both salt and ABA treatment only accounted for 9.75% of total differentially regulated genes. GO analysis showed that structural molecule activity, organelle part, membrane-enclosed lumen, reproduction, and reproductive process are enhanced by ABA but inhibited by salt stress. Conversely, immune system and multi-organism process were improved by salt but inhibited by ABA. Transcription regulator activity, enzyme regulator activity, and developmental process were significantly altered by ABA but were not affected by salt stress. Our study provides insights into how ThbZIP1 mediates ABA and salt stress response at the molecular level.
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spelling pubmed-43226382015-02-24 The regulatory network of ThbZIP1 in response to abscisic acid treatment Ji, Xiaoyu Liu, Guifeng Liu, Yujia Nie, Xianguang Zheng, Lei Wang, Yucheng Front Plant Sci Plant Science Previously, a bZIP transcription factor from Tamarix hispida, ThbZIP1, was characterized: plants overexpressing ThbZIP1 displayed improved salt stress tolerance but were sensitive to abscisic acid (ABA). In the current study, we further characterized the regulatory network of ThbZIP1 and the mechanism of ABA sensitivity mediated by ThbZIP1. An ABF transcription factor from T. hispida, ThABF1, directly regulates the expression of ThbZIP1. Microarray analysis identified 1662 and 1609 genes that were respectively significantly upregulated or downregulated by ThbZIP1 when exposed to ABA. Gene ontology (GO) analysis showed that the processes including “response to stimulus,” “catalytic activity,” “binding function,” and “metabolic process” were highly altered in ThbZIP1 expressing plants exposed to ABA. The gene expression in ThbZIP1 transformed plants were compared between exposed to ABA and salt on the genome scale. Genes differentially regulated by both salt and ABA treatment only accounted for 9.75% of total differentially regulated genes. GO analysis showed that structural molecule activity, organelle part, membrane-enclosed lumen, reproduction, and reproductive process are enhanced by ABA but inhibited by salt stress. Conversely, immune system and multi-organism process were improved by salt but inhibited by ABA. Transcription regulator activity, enzyme regulator activity, and developmental process were significantly altered by ABA but were not affected by salt stress. Our study provides insights into how ThbZIP1 mediates ABA and salt stress response at the molecular level. Frontiers Media S.A. 2015-02-10 /pmc/articles/PMC4322638/ /pubmed/25713576 http://dx.doi.org/10.3389/fpls.2015.00025 Text en Copyright © 2015 Ji, Liu, Liu, Nie, Zheng and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ji, Xiaoyu
Liu, Guifeng
Liu, Yujia
Nie, Xianguang
Zheng, Lei
Wang, Yucheng
The regulatory network of ThbZIP1 in response to abscisic acid treatment
title The regulatory network of ThbZIP1 in response to abscisic acid treatment
title_full The regulatory network of ThbZIP1 in response to abscisic acid treatment
title_fullStr The regulatory network of ThbZIP1 in response to abscisic acid treatment
title_full_unstemmed The regulatory network of ThbZIP1 in response to abscisic acid treatment
title_short The regulatory network of ThbZIP1 in response to abscisic acid treatment
title_sort regulatory network of thbzip1 in response to abscisic acid treatment
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322638/
https://www.ncbi.nlm.nih.gov/pubmed/25713576
http://dx.doi.org/10.3389/fpls.2015.00025
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