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Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism
Aim and Methods. In this study, we evaluated NOS and arginase activities and their regulation during Behçet disease, a systemic chronic inflammatory disorder with uncertain etiology. The peripheral blood mononuclear cells of 36 patients and 15 control samples (PBMC) were cultured in either RPMI 1640...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322663/ https://www.ncbi.nlm.nih.gov/pubmed/25692069 http://dx.doi.org/10.1155/2015/241738 |
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author | Belguendouz, Houda Lahmar-Belguendouz, Karima Messaoudene, Djamel Djeraba, Zineb Otmani, Fifi Hakem, Djennat Lahlou-Boukoffa, Ouided S. Youinou, Pierre Touil-Boukoffa, Chafia |
author_facet | Belguendouz, Houda Lahmar-Belguendouz, Karima Messaoudene, Djamel Djeraba, Zineb Otmani, Fifi Hakem, Djennat Lahlou-Boukoffa, Ouided S. Youinou, Pierre Touil-Boukoffa, Chafia |
author_sort | Belguendouz, Houda |
collection | PubMed |
description | Aim and Methods. In this study, we evaluated NOS and arginase activities and their regulation during Behçet disease, a systemic chronic inflammatory disorder with uncertain etiology. The peripheral blood mononuclear cells of 36 patients and 15 control samples (PBMC) were cultured in either RPMI 1640, MEM, or DMEM complemented with 10% of FBS and antibiotics. Cultures were performed with or without the control or patients plasma. Subsequent treatment contained anticytokines (IL-6, TGF-β), a mitogenic effector (PHA), or NOS modulators (L-NMMA, BH4). Culture supernatants were harvested after 24 h of incubation. NO and urea measurements were, respectively, performed by modified Griess and Berthelot methods. Results. Higher urea levels were found in patients' plasma compared to the control's (P < 0.05). NOS modulators induced inverted production profiles for NO and urea (P < 0.05). Their results differed depending on the clinical findings (P < 0.05). It was also found that cytokine neutralization induced different response profiles in patients as opposed to control cultures (P < 0.05). Conclusion. Our results suggest that arginases can compete with NOS2 for L-arginine during Behçet disease. Both enzymes are regulated by environmental cytokines and substrate availability. Furthermore, it seems that NOS/arginase balance is dependent on clinical expression. |
format | Online Article Text |
id | pubmed-4322663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43226632015-02-17 Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism Belguendouz, Houda Lahmar-Belguendouz, Karima Messaoudene, Djamel Djeraba, Zineb Otmani, Fifi Hakem, Djennat Lahlou-Boukoffa, Ouided S. Youinou, Pierre Touil-Boukoffa, Chafia Int J Inflam Clinical Study Aim and Methods. In this study, we evaluated NOS and arginase activities and their regulation during Behçet disease, a systemic chronic inflammatory disorder with uncertain etiology. The peripheral blood mononuclear cells of 36 patients and 15 control samples (PBMC) were cultured in either RPMI 1640, MEM, or DMEM complemented with 10% of FBS and antibiotics. Cultures were performed with or without the control or patients plasma. Subsequent treatment contained anticytokines (IL-6, TGF-β), a mitogenic effector (PHA), or NOS modulators (L-NMMA, BH4). Culture supernatants were harvested after 24 h of incubation. NO and urea measurements were, respectively, performed by modified Griess and Berthelot methods. Results. Higher urea levels were found in patients' plasma compared to the control's (P < 0.05). NOS modulators induced inverted production profiles for NO and urea (P < 0.05). Their results differed depending on the clinical findings (P < 0.05). It was also found that cytokine neutralization induced different response profiles in patients as opposed to control cultures (P < 0.05). Conclusion. Our results suggest that arginases can compete with NOS2 for L-arginine during Behçet disease. Both enzymes are regulated by environmental cytokines and substrate availability. Furthermore, it seems that NOS/arginase balance is dependent on clinical expression. Hindawi Publishing Corporation 2015 2015-01-27 /pmc/articles/PMC4322663/ /pubmed/25692069 http://dx.doi.org/10.1155/2015/241738 Text en Copyright © 2015 Houda Belguendouz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Study Belguendouz, Houda Lahmar-Belguendouz, Karima Messaoudene, Djamel Djeraba, Zineb Otmani, Fifi Hakem, Djennat Lahlou-Boukoffa, Ouided S. Youinou, Pierre Touil-Boukoffa, Chafia Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism |
title | Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism |
title_full | Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism |
title_fullStr | Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism |
title_full_unstemmed | Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism |
title_short | Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism |
title_sort | cytokines modulate the “immune-metabolism” interactions during behçet disease: effect on arginine metabolism |
topic | Clinical Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322663/ https://www.ncbi.nlm.nih.gov/pubmed/25692069 http://dx.doi.org/10.1155/2015/241738 |
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