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Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats
Objective. To evaluate the effect of GuiXiong Xiaoyi Wan (GXXYW) on the development of endometriosis in a rat model. Methods. Sprague-Dawley rats with surgically induced endometriosis were randomly treated with low-dose GXXYW, high-dose GXXYW, or vehicle (negative control) for 28 days. Immunohistoch...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322821/ https://www.ncbi.nlm.nih.gov/pubmed/25691906 http://dx.doi.org/10.1155/2015/208514 |
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author | Jin, Zhixing Wang, Li Zhu, Zhiling |
author_facet | Jin, Zhixing Wang, Li Zhu, Zhiling |
author_sort | Jin, Zhixing |
collection | PubMed |
description | Objective. To evaluate the effect of GuiXiong Xiaoyi Wan (GXXYW) on the development of endometriosis in a rat model. Methods. Sprague-Dawley rats with surgically induced endometriosis were randomly treated with low-dose GXXYW, high-dose GXXYW, or vehicle (negative control) for 28 days. Immunohistochemistry was used to assess cell proliferation in the lesions. The terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP biotin nick end labelling (TUNEL) method was performed to analyse the apoptosis induced by GuiXiong Xiaoyi Wan. The percentages of CD3+ lymphocytes, CD4+ lymphocytes, and CD8+ lymphocytes in the spleens of the rats were evaluated using flow cytometric analysis. Results. Treatment with GXXYW significantly decreased the lesion size, inhibited cell proliferation, and induced apoptosis in endometriotic tissue. The spleens of GXXYW-treated rats also demonstrated a significant increase in the percentage of CD4+ lymphocytes and a significant decrease in the percentage of CD8+ lymphocytes. Conclusions. These results suggest that, in a rat model, GXXYW may be effective in the suppression of the growth of endometriosis, possibly through the inhibition of cell proliferation, the induction of apoptosis of endometriotic cells, and the regulation of the immune system. |
format | Online Article Text |
id | pubmed-4322821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43228212015-02-17 Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats Jin, Zhixing Wang, Li Zhu, Zhiling Evid Based Complement Alternat Med Research Article Objective. To evaluate the effect of GuiXiong Xiaoyi Wan (GXXYW) on the development of endometriosis in a rat model. Methods. Sprague-Dawley rats with surgically induced endometriosis were randomly treated with low-dose GXXYW, high-dose GXXYW, or vehicle (negative control) for 28 days. Immunohistochemistry was used to assess cell proliferation in the lesions. The terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP biotin nick end labelling (TUNEL) method was performed to analyse the apoptosis induced by GuiXiong Xiaoyi Wan. The percentages of CD3+ lymphocytes, CD4+ lymphocytes, and CD8+ lymphocytes in the spleens of the rats were evaluated using flow cytometric analysis. Results. Treatment with GXXYW significantly decreased the lesion size, inhibited cell proliferation, and induced apoptosis in endometriotic tissue. The spleens of GXXYW-treated rats also demonstrated a significant increase in the percentage of CD4+ lymphocytes and a significant decrease in the percentage of CD8+ lymphocytes. Conclusions. These results suggest that, in a rat model, GXXYW may be effective in the suppression of the growth of endometriosis, possibly through the inhibition of cell proliferation, the induction of apoptosis of endometriotic cells, and the regulation of the immune system. Hindawi Publishing Corporation 2015 2015-01-27 /pmc/articles/PMC4322821/ /pubmed/25691906 http://dx.doi.org/10.1155/2015/208514 Text en Copyright © 2015 Zhixing Jin et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Jin, Zhixing Wang, Li Zhu, Zhiling Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats |
title | Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats |
title_full | Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats |
title_fullStr | Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats |
title_full_unstemmed | Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats |
title_short | Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats |
title_sort | effect of guixiong xiaoyi wan in treatment of endometriosis on rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322821/ https://www.ncbi.nlm.nih.gov/pubmed/25691906 http://dx.doi.org/10.1155/2015/208514 |
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