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ROS production, intracellular HSP70 levels and their relationship in human neutrophils: effects of age

ROS production and intracellular HSP70 levels were measured in human neutrophils for three age groups: young (20-59 years), elders (60-89 years) and nonagenarians (90 years and older). Elders showed higher levels of spontaneous intracellular ROS content compared with young and nonagenarian groups, w...

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Autores principales: Kovalenko, Elena I., Boyko, Anna A., Semenkov, Victor F., Lutsenko, Gennady V., Grechikhina, Maria V., Kanevskiy, Leonid M., Azhikina, Tatyana L., Telford, William G., Sapozhnikov, Alexander M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322963/
https://www.ncbi.nlm.nih.gov/pubmed/25514461
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author Kovalenko, Elena I.
Boyko, Anna A.
Semenkov, Victor F.
Lutsenko, Gennady V.
Grechikhina, Maria V.
Kanevskiy, Leonid M.
Azhikina, Tatyana L.
Telford, William G.
Sapozhnikov, Alexander M.
author_facet Kovalenko, Elena I.
Boyko, Anna A.
Semenkov, Victor F.
Lutsenko, Gennady V.
Grechikhina, Maria V.
Kanevskiy, Leonid M.
Azhikina, Tatyana L.
Telford, William G.
Sapozhnikov, Alexander M.
author_sort Kovalenko, Elena I.
collection PubMed
description ROS production and intracellular HSP70 levels were measured in human neutrophils for three age groups: young (20-59 years), elders (60-89 years) and nonagenarians (90 years and older). Elders showed higher levels of spontaneous intracellular ROS content compared with young and nonagenarian groups, which had similar intracellular ROS levels. Zymosan-induced (non-spontaneous) extracellular ROS levels were also similar for young and nonagenarians but were lower in elders. However, spontaneous extracellular ROS production increased continuously with age. Correlation analysis revealed positive relationships between HSP70 levels and zymosan-stimulated ROS production in the elder group. This was consistent with a promoting role for HSP70 in ROS-associated neutrophils response to pathogens. No positive correlation between ROS production and intracellular HSP70 levels was found for groups of young people and nonagenarians. In contrast, significant negative correlations of some ROS and HSP70 characteriscics were found for neutrophils from young people and nonagenarians. The observed difference in ROS and HSP70 correlations in elders and nonagenarians might be associated with an increased risk of mortality in older individuals less than 90 years old.
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spelling pubmed-43229632015-02-10 ROS production, intracellular HSP70 levels and their relationship in human neutrophils: effects of age Kovalenko, Elena I. Boyko, Anna A. Semenkov, Victor F. Lutsenko, Gennady V. Grechikhina, Maria V. Kanevskiy, Leonid M. Azhikina, Tatyana L. Telford, William G. Sapozhnikov, Alexander M. Oncotarget Gerotarget (Focus on Aging): Research Paper ROS production and intracellular HSP70 levels were measured in human neutrophils for three age groups: young (20-59 years), elders (60-89 years) and nonagenarians (90 years and older). Elders showed higher levels of spontaneous intracellular ROS content compared with young and nonagenarian groups, which had similar intracellular ROS levels. Zymosan-induced (non-spontaneous) extracellular ROS levels were also similar for young and nonagenarians but were lower in elders. However, spontaneous extracellular ROS production increased continuously with age. Correlation analysis revealed positive relationships between HSP70 levels and zymosan-stimulated ROS production in the elder group. This was consistent with a promoting role for HSP70 in ROS-associated neutrophils response to pathogens. No positive correlation between ROS production and intracellular HSP70 levels was found for groups of young people and nonagenarians. In contrast, significant negative correlations of some ROS and HSP70 characteriscics were found for neutrophils from young people and nonagenarians. The observed difference in ROS and HSP70 correlations in elders and nonagenarians might be associated with an increased risk of mortality in older individuals less than 90 years old. Impact Journals LLC 2014-12-03 /pmc/articles/PMC4322963/ /pubmed/25514461 Text en Copyright: © 2014 Kovalenko et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Gerotarget (Focus on Aging): Research Paper
Kovalenko, Elena I.
Boyko, Anna A.
Semenkov, Victor F.
Lutsenko, Gennady V.
Grechikhina, Maria V.
Kanevskiy, Leonid M.
Azhikina, Tatyana L.
Telford, William G.
Sapozhnikov, Alexander M.
ROS production, intracellular HSP70 levels and their relationship in human neutrophils: effects of age
title ROS production, intracellular HSP70 levels and their relationship in human neutrophils: effects of age
title_full ROS production, intracellular HSP70 levels and their relationship in human neutrophils: effects of age
title_fullStr ROS production, intracellular HSP70 levels and their relationship in human neutrophils: effects of age
title_full_unstemmed ROS production, intracellular HSP70 levels and their relationship in human neutrophils: effects of age
title_short ROS production, intracellular HSP70 levels and their relationship in human neutrophils: effects of age
title_sort ros production, intracellular hsp70 levels and their relationship in human neutrophils: effects of age
topic Gerotarget (Focus on Aging): Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322963/
https://www.ncbi.nlm.nih.gov/pubmed/25514461
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