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The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry
Ferritin is an iron-storage protein and its serum level is known to increase in the patient of with inflammation and malignant tumor. To further elucidate the difference between ferritins from normal human liver tissue and that of cancer cells, their sialic acids were analyzed. The Western blot anal...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japan Academy
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323041/ https://www.ncbi.nlm.nih.gov/pubmed/25792781 |
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author | ASAKAWA, Hideo SASABE, Masataka MIYAZAKI, Ryuunosuke MATSUDA, Haruo FUKAI, Fumio HANADA, Kazuki HIRANO, Hisashi TAKASAKI, Seiichi |
author_facet | ASAKAWA, Hideo SASABE, Masataka MIYAZAKI, Ryuunosuke MATSUDA, Haruo FUKAI, Fumio HANADA, Kazuki HIRANO, Hisashi TAKASAKI, Seiichi |
author_sort | ASAKAWA, Hideo |
collection | PubMed |
description | Ferritin is an iron-storage protein and its serum level is known to increase in the patient of with inflammation and malignant tumor. To further elucidate the difference between ferritins from normal human liver tissue and that of cancer cells, their sialic acids were analyzed. The Western blot analysis and the cytochemical staining using anti-NeuGc antiserum indicated that ferritins from the human hepatocarcinoma tissue and malignant K562 cells contain NeuGc, but that from the normal liver does not. The result was also confirmed by HPLC analysis and MALDI-TOF/MS analysis of sialic acids which were derivatized by the DMB method. It was also shown that the sialic acid content in hepatocarcinoma ferritin was much higher than that in the normal liver ferritin. These results suggest that normal and cancerous liver ferritins are qualitatively and quantitatively different in sialylation. In addition, K562 cells were shown to express NeuGc even if the cells were cultured in serum-free media which lack NeuGc. This is of interest from the current concept that expression of NeuGc in human cells is due to uptake and utilization of exogenous NeuGc. |
format | Online Article Text |
id | pubmed-4323041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Japan Academy |
record_format | MEDLINE/PubMed |
spelling | pubmed-43230412015-03-19 The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry ASAKAWA, Hideo SASABE, Masataka MIYAZAKI, Ryuunosuke MATSUDA, Haruo FUKAI, Fumio HANADA, Kazuki HIRANO, Hisashi TAKASAKI, Seiichi Proc Jpn Acad Ser B Phys Biol Sci Articles Ferritin is an iron-storage protein and its serum level is known to increase in the patient of with inflammation and malignant tumor. To further elucidate the difference between ferritins from normal human liver tissue and that of cancer cells, their sialic acids were analyzed. The Western blot analysis and the cytochemical staining using anti-NeuGc antiserum indicated that ferritins from the human hepatocarcinoma tissue and malignant K562 cells contain NeuGc, but that from the normal liver does not. The result was also confirmed by HPLC analysis and MALDI-TOF/MS analysis of sialic acids which were derivatized by the DMB method. It was also shown that the sialic acid content in hepatocarcinoma ferritin was much higher than that in the normal liver ferritin. These results suggest that normal and cancerous liver ferritins are qualitatively and quantitatively different in sialylation. In addition, K562 cells were shown to express NeuGc even if the cells were cultured in serum-free media which lack NeuGc. This is of interest from the current concept that expression of NeuGc in human cells is due to uptake and utilization of exogenous NeuGc. The Japan Academy 2006-07 /pmc/articles/PMC4323041/ /pubmed/25792781 Text en © 2006 The Japan Academy This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles ASAKAWA, Hideo SASABE, Masataka MIYAZAKI, Ryuunosuke MATSUDA, Haruo FUKAI, Fumio HANADA, Kazuki HIRANO, Hisashi TAKASAKI, Seiichi The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry |
title | The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry |
title_full | The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry |
title_fullStr | The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry |
title_full_unstemmed | The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry |
title_short | The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry |
title_sort | analysis of n-glycolylneuraminic acid(neugc) of hepatoma tissue and k562 cell ferritins using hplc and mass spectrometry |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323041/ https://www.ncbi.nlm.nih.gov/pubmed/25792781 |
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