Hepatic stellate cells: Three-dimensional structure, localization, heterogeneity and development

Hepatic stellate cells (HSCs) are vitamin-A storing collagen-producing cells in hepatic lobules. The three-dimensional structure of HSCs has been demonstrated with the Golgi method, the maceration method for scanning electron microscopy, and confocal laser scanning microscopy. Many thorn-like microprojections or spines extend from the subendothelial processes and make contacts with hepatocytes. One HSC entwines two or more sinusoids and about 20–40 hepatocytes to create a cellular unit, ‘the stellate cell unit’ or ‘stellon’. The Space of Disse is defined as the space between stellate cell-endothelial cell complex and hepatocytes. Intralobular heterogeneity of HSCs is assessed. HSCs develop from mesenchymal cells in the septum transversum. The developmental process of HSCs is reproduced partly in culture. In the lamprey abundant vitamin A is stored not only in HSCs, but in the fibroblast-like cells in the various other splanchnic organs. In vertebrates, the existence of both conventional fibroblast system in somatic tissues and vitamin A-storing cell system in splanchnic organs is suggested.