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Inhibitory effect of Curcuma purpurascens BI. rhizome on HT-29 colon cancer cells through mitochondrial-dependent apoptosis pathway

BACKGROUND: Curcuma purpurascens BI. (Zingiberaceae) commonly known as ‘Koneng Tinggang’ and ‘Temu Tis’ is a Javanese medicinal plant which has been used for numerous ailments and diseases in rural Javanese communities. In the present study, the apoptogenic activity of dichloromethane extract of Cur...

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Autores principales: Rouhollahi, Elham, Zorofchian Moghadamtousi, Soheil, Paydar, Mohammadjavad, Fadaeinasab, Mehran, Zahedifard, Maryam, Hajrezaie, Maryam, Abdalla Ahmed Hamdi, Omer, Yeng Looi, Chung, Ameen Abdulla, Mahmood, Awang, Khalijah, Mohamed, Zahurin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323059/
https://www.ncbi.nlm.nih.gov/pubmed/25652758
http://dx.doi.org/10.1186/s12906-015-0534-6
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author Rouhollahi, Elham
Zorofchian Moghadamtousi, Soheil
Paydar, Mohammadjavad
Fadaeinasab, Mehran
Zahedifard, Maryam
Hajrezaie, Maryam
Abdalla Ahmed Hamdi, Omer
Yeng Looi, Chung
Ameen Abdulla, Mahmood
Awang, Khalijah
Mohamed, Zahurin
author_facet Rouhollahi, Elham
Zorofchian Moghadamtousi, Soheil
Paydar, Mohammadjavad
Fadaeinasab, Mehran
Zahedifard, Maryam
Hajrezaie, Maryam
Abdalla Ahmed Hamdi, Omer
Yeng Looi, Chung
Ameen Abdulla, Mahmood
Awang, Khalijah
Mohamed, Zahurin
author_sort Rouhollahi, Elham
collection PubMed
description BACKGROUND: Curcuma purpurascens BI. (Zingiberaceae) commonly known as ‘Koneng Tinggang’ and ‘Temu Tis’ is a Javanese medicinal plant which has been used for numerous ailments and diseases in rural Javanese communities. In the present study, the apoptogenic activity of dichloromethane extract of Curcuma purpurascens BI. rhizome (DECPR) was investigated against HT-29 human colon cancer cells. METHODS: Acute toxicity study of DECPR was performed in Sprague–Dawley rats. Compounds of DECPR were analyzed by the gas chromatography–mass spectrometry–time of flight (GC-MS-TOF) analysis. Cytotoxic effect of DECPR on HT-29 cells was analyzed by MTT and lactate dehydrogenase (LDH) assays. Effects of DECPR on reactive oxygen species (ROS) formation and mitochondrial-initiated events were investigated using a high content screening system. The activities of the caspases were also measured using a fluorometric assay. The quantitative PCR analysis was carried out to examine the gene expression of Bax, Bcl-2 and Bcl-xl proteins. RESULTS: The in vivo acute toxicity study of DECPR on rats showed the safety of this extract at the highest dose of 5 g/kg. The GC-MS-TOF analysis of DECPR detected turmerone as the major compound in dichloromethane extract. IC(50) value of DECPR towards HT-29 cells after 24 h treatment was found to be 7.79 ± 0.54 μg/mL. In addition, DECPR induced LDH release and ROS generation in HT-29 cells through a mechanism involving nuclear fragmentation and cytoskeletal rearrangement. The mitochondrial-initiated events, including collapse in mitochondrial membrane potential and cytochrome c leakage was also triggered by DECPR treatment. Initiator caspase-9 and executioner caspase-3 was dose-dependently activated by DECPR. The quantitative PCR analysis on the mRNA expression of Bcl-2 family of proteins showed a significant up-regulation of Bax associated with down-regulation in Bcl-2 and Bcl-xl mRNA expression. CONCLUSIONS: The findings presented in the current study showed that DECP suppressed the proliferation of HT-29 colon cancer cells and triggered the induction of apoptosis through mitochondrial-dependent pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-015-0534-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-43230592015-02-11 Inhibitory effect of Curcuma purpurascens BI. rhizome on HT-29 colon cancer cells through mitochondrial-dependent apoptosis pathway Rouhollahi, Elham Zorofchian Moghadamtousi, Soheil Paydar, Mohammadjavad Fadaeinasab, Mehran Zahedifard, Maryam Hajrezaie, Maryam Abdalla Ahmed Hamdi, Omer Yeng Looi, Chung Ameen Abdulla, Mahmood Awang, Khalijah Mohamed, Zahurin BMC Complement Altern Med Research Article BACKGROUND: Curcuma purpurascens BI. (Zingiberaceae) commonly known as ‘Koneng Tinggang’ and ‘Temu Tis’ is a Javanese medicinal plant which has been used for numerous ailments and diseases in rural Javanese communities. In the present study, the apoptogenic activity of dichloromethane extract of Curcuma purpurascens BI. rhizome (DECPR) was investigated against HT-29 human colon cancer cells. METHODS: Acute toxicity study of DECPR was performed in Sprague–Dawley rats. Compounds of DECPR were analyzed by the gas chromatography–mass spectrometry–time of flight (GC-MS-TOF) analysis. Cytotoxic effect of DECPR on HT-29 cells was analyzed by MTT and lactate dehydrogenase (LDH) assays. Effects of DECPR on reactive oxygen species (ROS) formation and mitochondrial-initiated events were investigated using a high content screening system. The activities of the caspases were also measured using a fluorometric assay. The quantitative PCR analysis was carried out to examine the gene expression of Bax, Bcl-2 and Bcl-xl proteins. RESULTS: The in vivo acute toxicity study of DECPR on rats showed the safety of this extract at the highest dose of 5 g/kg. The GC-MS-TOF analysis of DECPR detected turmerone as the major compound in dichloromethane extract. IC(50) value of DECPR towards HT-29 cells after 24 h treatment was found to be 7.79 ± 0.54 μg/mL. In addition, DECPR induced LDH release and ROS generation in HT-29 cells through a mechanism involving nuclear fragmentation and cytoskeletal rearrangement. The mitochondrial-initiated events, including collapse in mitochondrial membrane potential and cytochrome c leakage was also triggered by DECPR treatment. Initiator caspase-9 and executioner caspase-3 was dose-dependently activated by DECPR. The quantitative PCR analysis on the mRNA expression of Bcl-2 family of proteins showed a significant up-regulation of Bax associated with down-regulation in Bcl-2 and Bcl-xl mRNA expression. CONCLUSIONS: The findings presented in the current study showed that DECP suppressed the proliferation of HT-29 colon cancer cells and triggered the induction of apoptosis through mitochondrial-dependent pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-015-0534-6) contains supplementary material, which is available to authorized users. BioMed Central 2015-02-05 /pmc/articles/PMC4323059/ /pubmed/25652758 http://dx.doi.org/10.1186/s12906-015-0534-6 Text en © Rouhollahi et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Rouhollahi, Elham
Zorofchian Moghadamtousi, Soheil
Paydar, Mohammadjavad
Fadaeinasab, Mehran
Zahedifard, Maryam
Hajrezaie, Maryam
Abdalla Ahmed Hamdi, Omer
Yeng Looi, Chung
Ameen Abdulla, Mahmood
Awang, Khalijah
Mohamed, Zahurin
Inhibitory effect of Curcuma purpurascens BI. rhizome on HT-29 colon cancer cells through mitochondrial-dependent apoptosis pathway
title Inhibitory effect of Curcuma purpurascens BI. rhizome on HT-29 colon cancer cells through mitochondrial-dependent apoptosis pathway
title_full Inhibitory effect of Curcuma purpurascens BI. rhizome on HT-29 colon cancer cells through mitochondrial-dependent apoptosis pathway
title_fullStr Inhibitory effect of Curcuma purpurascens BI. rhizome on HT-29 colon cancer cells through mitochondrial-dependent apoptosis pathway
title_full_unstemmed Inhibitory effect of Curcuma purpurascens BI. rhizome on HT-29 colon cancer cells through mitochondrial-dependent apoptosis pathway
title_short Inhibitory effect of Curcuma purpurascens BI. rhizome on HT-29 colon cancer cells through mitochondrial-dependent apoptosis pathway
title_sort inhibitory effect of curcuma purpurascens bi. rhizome on ht-29 colon cancer cells through mitochondrial-dependent apoptosis pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323059/
https://www.ncbi.nlm.nih.gov/pubmed/25652758
http://dx.doi.org/10.1186/s12906-015-0534-6
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