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Characterization and comparison of bacterial communities in benign vocal fold lesions

BACKGROUND: Benign vocal fold lesions, including cysts, nodules, polyps, and Reinke’s edema, are common causes of hoarseness and subsequent voice disorders. Given the prevalence of these lesions, disease etiology and pathophysiology remain unclear and their microbiota has not been studied to date se...

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Autores principales: Hanshew, Alissa S, Jetté, Marie E, Thibeault, Susan L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323261/
https://www.ncbi.nlm.nih.gov/pubmed/25671105
http://dx.doi.org/10.1186/2049-2618-2-43
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author Hanshew, Alissa S
Jetté, Marie E
Thibeault, Susan L
author_facet Hanshew, Alissa S
Jetté, Marie E
Thibeault, Susan L
author_sort Hanshew, Alissa S
collection PubMed
description BACKGROUND: Benign vocal fold lesions, including cysts, nodules, polyps, and Reinke’s edema, are common causes of hoarseness and subsequent voice disorders. Given the prevalence of these lesions, disease etiology and pathophysiology remain unclear and their microbiota has not been studied to date secondary to the paucity of available biopsies for investigation. We sought to characterize and compare the bacterial communities in biopsies of cysts, nodules, polyps, and Reinke’s edema collected from patients in Germany and Wisconsin. These samples were then compared to the communities found in healthy saliva and throat samples from the Human Microbiome Project (HMP). RESULTS: 454 pyrosequencing of the V3–V5 regions of the 16S rRNA gene revealed five phyla that explained most of the bacterial diversity, including Firmicutes (73.8%), Proteobacteria (12.7%), Bacteroidetes (9.2%), Actinobacteria (2.1%), and Fusobacteria (1.9%). Every lesion sample, regardless of diagnosis, had operational taxonomic units (OTUs) identified as Streptococcus, with a mean abundance of 68.7%. Most of the lesions, 31 out of 44, were indistinguishable in a principal coordinates analysis (PCoA) due to dominance by OTUs phylogenetically similar to Streptococcus pseudopneumoniae. Thirteen lesions not dominated by S. pseudopneumoniae were more similar to HMP throat and saliva samples, though 12 of them contained Pseudomonas, which was not present in any of the HMP samples. Community structure and abundance could not be correlated with lesion diagnosis or any other documented patient factor, including age, sex, or country of origin. CONCLUSIONS: Dominance by S. pseudopneumoniae could be a factor in disease etiology, as could the presence of Pseudomonas in some samples. Likewise, decreased diversity, as compared to healthy saliva and throat samples, may be associated with disease, similar to disease models in other mucosal sites. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2049-2618-2-43) contains supplementary material, which is available to authorized users.
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spelling pubmed-43232612015-02-11 Characterization and comparison of bacterial communities in benign vocal fold lesions Hanshew, Alissa S Jetté, Marie E Thibeault, Susan L Microbiome Research BACKGROUND: Benign vocal fold lesions, including cysts, nodules, polyps, and Reinke’s edema, are common causes of hoarseness and subsequent voice disorders. Given the prevalence of these lesions, disease etiology and pathophysiology remain unclear and their microbiota has not been studied to date secondary to the paucity of available biopsies for investigation. We sought to characterize and compare the bacterial communities in biopsies of cysts, nodules, polyps, and Reinke’s edema collected from patients in Germany and Wisconsin. These samples were then compared to the communities found in healthy saliva and throat samples from the Human Microbiome Project (HMP). RESULTS: 454 pyrosequencing of the V3–V5 regions of the 16S rRNA gene revealed five phyla that explained most of the bacterial diversity, including Firmicutes (73.8%), Proteobacteria (12.7%), Bacteroidetes (9.2%), Actinobacteria (2.1%), and Fusobacteria (1.9%). Every lesion sample, regardless of diagnosis, had operational taxonomic units (OTUs) identified as Streptococcus, with a mean abundance of 68.7%. Most of the lesions, 31 out of 44, were indistinguishable in a principal coordinates analysis (PCoA) due to dominance by OTUs phylogenetically similar to Streptococcus pseudopneumoniae. Thirteen lesions not dominated by S. pseudopneumoniae were more similar to HMP throat and saliva samples, though 12 of them contained Pseudomonas, which was not present in any of the HMP samples. Community structure and abundance could not be correlated with lesion diagnosis or any other documented patient factor, including age, sex, or country of origin. CONCLUSIONS: Dominance by S. pseudopneumoniae could be a factor in disease etiology, as could the presence of Pseudomonas in some samples. Likewise, decreased diversity, as compared to healthy saliva and throat samples, may be associated with disease, similar to disease models in other mucosal sites. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2049-2618-2-43) contains supplementary material, which is available to authorized users. BioMed Central 2014-12-08 /pmc/articles/PMC4323261/ /pubmed/25671105 http://dx.doi.org/10.1186/2049-2618-2-43 Text en © Hanshew et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Hanshew, Alissa S
Jetté, Marie E
Thibeault, Susan L
Characterization and comparison of bacterial communities in benign vocal fold lesions
title Characterization and comparison of bacterial communities in benign vocal fold lesions
title_full Characterization and comparison of bacterial communities in benign vocal fold lesions
title_fullStr Characterization and comparison of bacterial communities in benign vocal fold lesions
title_full_unstemmed Characterization and comparison of bacterial communities in benign vocal fold lesions
title_short Characterization and comparison of bacterial communities in benign vocal fold lesions
title_sort characterization and comparison of bacterial communities in benign vocal fold lesions
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323261/
https://www.ncbi.nlm.nih.gov/pubmed/25671105
http://dx.doi.org/10.1186/2049-2618-2-43
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