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Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2
A bacterial isolate capable of utilizing tributyltin chloride (TBTCl) as sole carbon source was isolated from estuarine sediments of west coast of India and identified as Pseudomonas stutzeri based on biochemical tests and Fatty acid methyl ester (FAME) analysis. This isolate was designated as strai...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323296/ https://www.ncbi.nlm.nih.gov/pubmed/25763027 |
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author | Khanolkar, Dnyanada S. Naik, Milind Mohan Dubey, Santosh Kumar |
author_facet | Khanolkar, Dnyanada S. Naik, Milind Mohan Dubey, Santosh Kumar |
author_sort | Khanolkar, Dnyanada S. |
collection | PubMed |
description | A bacterial isolate capable of utilizing tributyltin chloride (TBTCl) as sole carbon source was isolated from estuarine sediments of west coast of India and identified as Pseudomonas stutzeri based on biochemical tests and Fatty acid methyl ester (FAME) analysis. This isolate was designated as strain DN2. Although this bacterial isolate could resist up to 3 mM TBTCl level, it showed maximum growth at 2 mM TBTCl in mineral salt medium (MSM). Pseudomonas stutzeri DN2 exposed to 2 mM TBTCl revealed significant alteration in cell morphology as elongation and shrinkage in cell size along with roughness of cell surface. FTIR and NMR analysis of TBTCl degradation product extracted using chloroform and purified using column chromatography clearly revealed biotransformation of TBTCl into Dibutyltin dichloride (DBTCl(2)) through debutylation process. Therefore, Pseudomonas stutzeri strain DN2 may be used as a potential bacterial strain for bioremediation of TBTCl contaminated aquatic environmental sites. |
format | Online Article Text |
id | pubmed-4323296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-43232962015-04-04 Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2 Khanolkar, Dnyanada S. Naik, Milind Mohan Dubey, Santosh Kumar Braz J Microbiol Environmental Microbiology A bacterial isolate capable of utilizing tributyltin chloride (TBTCl) as sole carbon source was isolated from estuarine sediments of west coast of India and identified as Pseudomonas stutzeri based on biochemical tests and Fatty acid methyl ester (FAME) analysis. This isolate was designated as strain DN2. Although this bacterial isolate could resist up to 3 mM TBTCl level, it showed maximum growth at 2 mM TBTCl in mineral salt medium (MSM). Pseudomonas stutzeri DN2 exposed to 2 mM TBTCl revealed significant alteration in cell morphology as elongation and shrinkage in cell size along with roughness of cell surface. FTIR and NMR analysis of TBTCl degradation product extracted using chloroform and purified using column chromatography clearly revealed biotransformation of TBTCl into Dibutyltin dichloride (DBTCl(2)) through debutylation process. Therefore, Pseudomonas stutzeri strain DN2 may be used as a potential bacterial strain for bioremediation of TBTCl contaminated aquatic environmental sites. Sociedade Brasileira de Microbiologia 2015-03-04 /pmc/articles/PMC4323296/ /pubmed/25763027 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Environmental Microbiology Khanolkar, Dnyanada S. Naik, Milind Mohan Dubey, Santosh Kumar Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2 |
title | Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2 |
title_full | Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2 |
title_fullStr | Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2 |
title_full_unstemmed | Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2 |
title_short | Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2 |
title_sort | biotransformation of tributyltin chloride by pseudomonas stutzeri strain dn2 |
topic | Environmental Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323296/ https://www.ncbi.nlm.nih.gov/pubmed/25763027 |
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