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New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract
The use of hemicellulosic hydrolysates in bioprocesses requires supplementation as to ensure the best fermentative performance of microorganisms. However, in light of conflicting data in the literature, it is necessary to establish an inexpensive and applicable medium for the development of bioproce...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323325/ https://www.ncbi.nlm.nih.gov/pubmed/25763056 |
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author | da Silva, Debora Danielle Virginio Cândido, Elisangela de Jesus de Arruda, Priscila Vaz da Silva, Silvio Silvério Felipe, Maria das Graças de Almeida |
author_facet | da Silva, Debora Danielle Virginio Cândido, Elisangela de Jesus de Arruda, Priscila Vaz da Silva, Silvio Silvério Felipe, Maria das Graças de Almeida |
author_sort | da Silva, Debora Danielle Virginio |
collection | PubMed |
description | The use of hemicellulosic hydrolysates in bioprocesses requires supplementation as to ensure the best fermentative performance of microorganisms. However, in light of conflicting data in the literature, it is necessary to establish an inexpensive and applicable medium for the development of bioprocesses. This paper evaluates the fermentative performance of Scheffersomyces (Pichia) stipitis and Candida guilliermondii growth in sugarcane bagasse hemicellulosic hydrolysate supplemented with different nitrogen sources including rice bran extract, an important by-product of agroindustry and source of vitamins and amino acids. Experiments were carried out with hydrolysate supplemented with rice bran extract and (NH(4))(2)SO(4); peptone and yeast extract; (NH(4))(2)SO(4), peptone and yeast extract and non-supplemented hydrolysate as a control. S. stipitis produced only ethanol, while C. guilliermondii produced xylitol as the main product and ethanol as by-product. Maximum ethanol production by S. stipitis was observed when sugarcane bagasse hemicellulosic hydrolysate was supplemented with (NH(4))(2)SO(4), peptone and yeast extract. Differently, the maximum xylitol formation by C. guilliermondii was obtained by employing hydrolysate supplemented with (NH(4))(2)SO(4) and rice bran extract. Together, these findings indicate that: a) for both yeasts (NH(4))(2)SO(4) was required as an inorganic nitrogen source to supplement sugarcane bagasse hydrolysate; b) for S. stipitis, sugarcane hemicellulosic hydrolysate must be supplemented with peptone and yeast extract as organic nitrogen source; and: c) for C. guilliermondii, it must be supplemented with rice bran extract. The present study designed a fermentation medium employing hemicellulosic hydrolysate and provides a basis for studies about value-added products as ethanol and xylitol from lignocellulosic materials. |
format | Online Article Text |
id | pubmed-4323325 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-43233252015-04-04 New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract da Silva, Debora Danielle Virginio Cândido, Elisangela de Jesus de Arruda, Priscila Vaz da Silva, Silvio Silvério Felipe, Maria das Graças de Almeida Braz J Microbiol Research Paper The use of hemicellulosic hydrolysates in bioprocesses requires supplementation as to ensure the best fermentative performance of microorganisms. However, in light of conflicting data in the literature, it is necessary to establish an inexpensive and applicable medium for the development of bioprocesses. This paper evaluates the fermentative performance of Scheffersomyces (Pichia) stipitis and Candida guilliermondii growth in sugarcane bagasse hemicellulosic hydrolysate supplemented with different nitrogen sources including rice bran extract, an important by-product of agroindustry and source of vitamins and amino acids. Experiments were carried out with hydrolysate supplemented with rice bran extract and (NH(4))(2)SO(4); peptone and yeast extract; (NH(4))(2)SO(4), peptone and yeast extract and non-supplemented hydrolysate as a control. S. stipitis produced only ethanol, while C. guilliermondii produced xylitol as the main product and ethanol as by-product. Maximum ethanol production by S. stipitis was observed when sugarcane bagasse hemicellulosic hydrolysate was supplemented with (NH(4))(2)SO(4), peptone and yeast extract. Differently, the maximum xylitol formation by C. guilliermondii was obtained by employing hydrolysate supplemented with (NH(4))(2)SO(4) and rice bran extract. Together, these findings indicate that: a) for both yeasts (NH(4))(2)SO(4) was required as an inorganic nitrogen source to supplement sugarcane bagasse hydrolysate; b) for S. stipitis, sugarcane hemicellulosic hydrolysate must be supplemented with peptone and yeast extract as organic nitrogen source; and: c) for C. guilliermondii, it must be supplemented with rice bran extract. The present study designed a fermentation medium employing hemicellulosic hydrolysate and provides a basis for studies about value-added products as ethanol and xylitol from lignocellulosic materials. Sociedade Brasileira de Microbiologia 2015-03-04 /pmc/articles/PMC4323325/ /pubmed/25763056 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Research Paper da Silva, Debora Danielle Virginio Cândido, Elisangela de Jesus de Arruda, Priscila Vaz da Silva, Silvio Silvério Felipe, Maria das Graças de Almeida New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract |
title | New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract |
title_full | New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract |
title_fullStr | New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract |
title_full_unstemmed | New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract |
title_short | New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract |
title_sort | new cultive medium for bioconversion of c5 fraction from sugarcane bagasse using rice bran extract |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323325/ https://www.ncbi.nlm.nih.gov/pubmed/25763056 |
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