Cargando…

Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels

Filamentous fungi are considered to be the most important group of microorganisms for the production of plant cell wall degrading enzymes (CWDE), in solid state fermentations. In this study, two fungal strains Aspergillus niger MS23 and Aspergillus terreus MS105 were screened for plant CWDE such as...

Descripción completa

Detalles Bibliográficos
Autores principales: Rehman, Shazia, Aslam, Hina, Ahmad, Aqeel, Khan, Shakeel Ahmed, Sohail, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323327/
https://www.ncbi.nlm.nih.gov/pubmed/25763058
_version_ 1782356530944802816
author Rehman, Shazia
Aslam, Hina
Ahmad, Aqeel
Khan, Shakeel Ahmed
Sohail, Muhammad
author_facet Rehman, Shazia
Aslam, Hina
Ahmad, Aqeel
Khan, Shakeel Ahmed
Sohail, Muhammad
author_sort Rehman, Shazia
collection PubMed
description Filamentous fungi are considered to be the most important group of microorganisms for the production of plant cell wall degrading enzymes (CWDE), in solid state fermentations. In this study, two fungal strains Aspergillus niger MS23 and Aspergillus terreus MS105 were screened for plant CWDE such as amylase, pectinase, xylanase and cellulases (β-glucosidase, endoglucanase and filterpaperase) using a novel substrate, Banana Peels (BP) for SSF process. This is the first study, to the best of our knowledge, to use BP as SSF substrate for plant CWDE production by co-culture of fungal strains. The titers of pectinase were significantly improved in co-culture compared to mono-culture. Furthermore, the enzyme preparations obtained from monoculture and co-culture were used to study the hydrolysis of BP along with some crude and purified substrates. It was observed that the enzymatic hydrolysis of different crude and purified substrates accomplished after 26 h of incubation, where pectin was maximally hydrolyzed by the enzyme preparations of mono and co-culture. Along with purified substrates, crude materials were also proved to be efficiently degraded by the cocktail of the CWDE. These results demonstrated that banana peels may be a potential substrate in solid-state fermentation for the production of plant cell wall degrading enzymes to be used for improving various biotechnological and industrial processes.
format Online
Article
Text
id pubmed-4323327
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-43233272015-04-04 Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels Rehman, Shazia Aslam, Hina Ahmad, Aqeel Khan, Shakeel Ahmed Sohail, Muhammad Braz J Microbiol Industrial Microbiology Filamentous fungi are considered to be the most important group of microorganisms for the production of plant cell wall degrading enzymes (CWDE), in solid state fermentations. In this study, two fungal strains Aspergillus niger MS23 and Aspergillus terreus MS105 were screened for plant CWDE such as amylase, pectinase, xylanase and cellulases (β-glucosidase, endoglucanase and filterpaperase) using a novel substrate, Banana Peels (BP) for SSF process. This is the first study, to the best of our knowledge, to use BP as SSF substrate for plant CWDE production by co-culture of fungal strains. The titers of pectinase were significantly improved in co-culture compared to mono-culture. Furthermore, the enzyme preparations obtained from monoculture and co-culture were used to study the hydrolysis of BP along with some crude and purified substrates. It was observed that the enzymatic hydrolysis of different crude and purified substrates accomplished after 26 h of incubation, where pectin was maximally hydrolyzed by the enzyme preparations of mono and co-culture. Along with purified substrates, crude materials were also proved to be efficiently degraded by the cocktail of the CWDE. These results demonstrated that banana peels may be a potential substrate in solid-state fermentation for the production of plant cell wall degrading enzymes to be used for improving various biotechnological and industrial processes. Sociedade Brasileira de Microbiologia 2015-03-04 /pmc/articles/PMC4323327/ /pubmed/25763058 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Industrial Microbiology
Rehman, Shazia
Aslam, Hina
Ahmad, Aqeel
Khan, Shakeel Ahmed
Sohail, Muhammad
Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels
title Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels
title_full Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels
title_fullStr Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels
title_full_unstemmed Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels
title_short Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels
title_sort production of plant cell wall degrading enzymes by monoculture and co-culture of aspergillus niger and aspergillus terreus under ssf of banana peels
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323327/
https://www.ncbi.nlm.nih.gov/pubmed/25763058
work_keys_str_mv AT rehmanshazia productionofplantcellwalldegradingenzymesbymonocultureandcocultureofaspergillusnigerandaspergillusterreusunderssfofbananapeels
AT aslamhina productionofplantcellwalldegradingenzymesbymonocultureandcocultureofaspergillusnigerandaspergillusterreusunderssfofbananapeels
AT ahmadaqeel productionofplantcellwalldegradingenzymesbymonocultureandcocultureofaspergillusnigerandaspergillusterreusunderssfofbananapeels
AT khanshakeelahmed productionofplantcellwalldegradingenzymesbymonocultureandcocultureofaspergillusnigerandaspergillusterreusunderssfofbananapeels
AT sohailmuhammad productionofplantcellwalldegradingenzymesbymonocultureandcocultureofaspergillusnigerandaspergillusterreusunderssfofbananapeels