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Molecular characterization of the first leptospires isolated from goats in Brazil
Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences rev...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323332/ https://www.ncbi.nlm.nih.gov/pubmed/25763063 |
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author | Lilenbaum, Walter Kremer, Frederico Ristow, Paula Dellagostin, Odir Bourhy, Pascale Hartskeerl, Rudy Vasconcellos, Silvio |
author_facet | Lilenbaum, Walter Kremer, Frederico Ristow, Paula Dellagostin, Odir Bourhy, Pascale Hartskeerl, Rudy Vasconcellos, Silvio |
author_sort | Lilenbaum, Walter |
collection | PubMed |
description | Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of caprine origin as closely related but not identical to serovar Bananal, and constitute a new type named Carioca. |
format | Online Article Text |
id | pubmed-4323332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-43233322015-04-04 Molecular characterization of the first leptospires isolated from goats in Brazil Lilenbaum, Walter Kremer, Frederico Ristow, Paula Dellagostin, Odir Bourhy, Pascale Hartskeerl, Rudy Vasconcellos, Silvio Braz J Microbiol Veterinary Microbiology Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of caprine origin as closely related but not identical to serovar Bananal, and constitute a new type named Carioca. Sociedade Brasileira de Microbiologia 2015-03-04 /pmc/articles/PMC4323332/ /pubmed/25763063 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Veterinary Microbiology Lilenbaum, Walter Kremer, Frederico Ristow, Paula Dellagostin, Odir Bourhy, Pascale Hartskeerl, Rudy Vasconcellos, Silvio Molecular characterization of the first leptospires isolated from goats in Brazil |
title | Molecular characterization of the first leptospires isolated from goats in Brazil |
title_full | Molecular characterization of the first leptospires isolated from goats in Brazil |
title_fullStr | Molecular characterization of the first leptospires isolated from goats in Brazil |
title_full_unstemmed | Molecular characterization of the first leptospires isolated from goats in Brazil |
title_short | Molecular characterization of the first leptospires isolated from goats in Brazil |
title_sort | molecular characterization of the first leptospires isolated from goats in brazil |
topic | Veterinary Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323332/ https://www.ncbi.nlm.nih.gov/pubmed/25763063 |
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