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Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) are considered a potential tool for cell based regenerative therapy due to their immunomodulatory property, differentiation potentials, trophic activity as well as large donor pool. Poor engraftment and short term survival of transplanted MSCs are recognized as major li...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323372/ https://www.ncbi.nlm.nih.gov/pubmed/25678851 http://dx.doi.org/10.7150/ijbs.10567 |
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author | Haque, Nazmul Kasim, Noor Hayaty Abu Rahman, Mohammad Tariqur |
author_facet | Haque, Nazmul Kasim, Noor Hayaty Abu Rahman, Mohammad Tariqur |
author_sort | Haque, Nazmul |
collection | PubMed |
description | Mesenchymal stem cells (MSCs) are considered a potential tool for cell based regenerative therapy due to their immunomodulatory property, differentiation potentials, trophic activity as well as large donor pool. Poor engraftment and short term survival of transplanted MSCs are recognized as major limitations which were linked to early cellular ageing, loss of chemokine markers during ex vivo expansion, and hyper-immunogenicity to xeno-contaminated MSCs. These problems can be minimized by ex vivo expansion of MSCs in hypoxic culture condition using well defined or xeno-free media i.e., media supplemented with growth factors, human serum or platelet lysate. In addition to ex vivo expansion in hypoxic culture condition using well defined media, this review article describes the potentials of transient adaptation of expanded MSCs in autologous serum supplemented medium prior to transplantation for long term regenerative benefits. Such transient adaptation in autologous serum supplemented medium may help to increase chemokine receptor expression and tissue specific differentiation of ex vivo expanded MSCs, thus would provide long term regenerative benefits. |
format | Online Article Text |
id | pubmed-4323372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-43233722015-02-12 Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells Haque, Nazmul Kasim, Noor Hayaty Abu Rahman, Mohammad Tariqur Int J Biol Sci Review Mesenchymal stem cells (MSCs) are considered a potential tool for cell based regenerative therapy due to their immunomodulatory property, differentiation potentials, trophic activity as well as large donor pool. Poor engraftment and short term survival of transplanted MSCs are recognized as major limitations which were linked to early cellular ageing, loss of chemokine markers during ex vivo expansion, and hyper-immunogenicity to xeno-contaminated MSCs. These problems can be minimized by ex vivo expansion of MSCs in hypoxic culture condition using well defined or xeno-free media i.e., media supplemented with growth factors, human serum or platelet lysate. In addition to ex vivo expansion in hypoxic culture condition using well defined media, this review article describes the potentials of transient adaptation of expanded MSCs in autologous serum supplemented medium prior to transplantation for long term regenerative benefits. Such transient adaptation in autologous serum supplemented medium may help to increase chemokine receptor expression and tissue specific differentiation of ex vivo expanded MSCs, thus would provide long term regenerative benefits. Ivyspring International Publisher 2015-02-05 /pmc/articles/PMC4323372/ /pubmed/25678851 http://dx.doi.org/10.7150/ijbs.10567 Text en © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. |
spellingShingle | Review Haque, Nazmul Kasim, Noor Hayaty Abu Rahman, Mohammad Tariqur Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells |
title | Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells |
title_full | Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells |
title_fullStr | Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells |
title_full_unstemmed | Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells |
title_short | Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells |
title_sort | optimization of pre-transplantation conditions to enhance the efficacy of mesenchymal stem cells |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323372/ https://www.ncbi.nlm.nih.gov/pubmed/25678851 http://dx.doi.org/10.7150/ijbs.10567 |
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