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Gadd45b is a Novel Mediator of Neuronal Apoptosis in Ischemic Stroke
Apoptosis plays an essential role in ischemic stroke pathogenesis. Research on the process of neuronal apoptosis in models of ischemic brain injury seems promising. The role of growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) in brain ischemia has not been fully examined to date. Thi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323375/ https://www.ncbi.nlm.nih.gov/pubmed/25678854 http://dx.doi.org/10.7150/ijbs.9813 |
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author | Liu, Bin Zhang, Yan-hong Jiang, Ying Li, Long-ling Chen, Qian He, Guo-qian Tan, Xiao-dan Li, Chang-qing |
author_facet | Liu, Bin Zhang, Yan-hong Jiang, Ying Li, Long-ling Chen, Qian He, Guo-qian Tan, Xiao-dan Li, Chang-qing |
author_sort | Liu, Bin |
collection | PubMed |
description | Apoptosis plays an essential role in ischemic stroke pathogenesis. Research on the process of neuronal apoptosis in models of ischemic brain injury seems promising. The role of growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) in brain ischemia has not been fully examined to date. This study aims to investigate the function of Gadd45b in ischemia-induced apoptosis. Adult male Sprague-Dawley rats were subjected to brain ischemia by middle cerebral artery occlusion (MCAO). RNA interference (RNAi) system, which is mediated by a lentiviral vector (LV), was stereotaxically injected into the ipsilateral lateral ventricle to knockdown Gadd45b expression. Neurologic scores and infarct volumes were assessed 24 h after reperfusion. Apoptosis-related molecules were studied using immunohistochemistry and Western blot analysis. We found that Gadd45b-RNAi significantly increased infarct volumes and worsened the outcome of transient focal cerebral ischemia. Gadd45b-RNAi also significantly increased neuronal apoptosis as indicated by increased levels of Bax and active caspase-3, and decreased levels of Bcl-2. These results indicate that Gadd45b is a beneficial mediator of neuronal apoptosis. |
format | Online Article Text |
id | pubmed-4323375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-43233752015-02-12 Gadd45b is a Novel Mediator of Neuronal Apoptosis in Ischemic Stroke Liu, Bin Zhang, Yan-hong Jiang, Ying Li, Long-ling Chen, Qian He, Guo-qian Tan, Xiao-dan Li, Chang-qing Int J Biol Sci Research Paper Apoptosis plays an essential role in ischemic stroke pathogenesis. Research on the process of neuronal apoptosis in models of ischemic brain injury seems promising. The role of growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) in brain ischemia has not been fully examined to date. This study aims to investigate the function of Gadd45b in ischemia-induced apoptosis. Adult male Sprague-Dawley rats were subjected to brain ischemia by middle cerebral artery occlusion (MCAO). RNA interference (RNAi) system, which is mediated by a lentiviral vector (LV), was stereotaxically injected into the ipsilateral lateral ventricle to knockdown Gadd45b expression. Neurologic scores and infarct volumes were assessed 24 h after reperfusion. Apoptosis-related molecules were studied using immunohistochemistry and Western blot analysis. We found that Gadd45b-RNAi significantly increased infarct volumes and worsened the outcome of transient focal cerebral ischemia. Gadd45b-RNAi also significantly increased neuronal apoptosis as indicated by increased levels of Bax and active caspase-3, and decreased levels of Bcl-2. These results indicate that Gadd45b is a beneficial mediator of neuronal apoptosis. Ivyspring International Publisher 2015-02-08 /pmc/articles/PMC4323375/ /pubmed/25678854 http://dx.doi.org/10.7150/ijbs.9813 Text en © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. |
spellingShingle | Research Paper Liu, Bin Zhang, Yan-hong Jiang, Ying Li, Long-ling Chen, Qian He, Guo-qian Tan, Xiao-dan Li, Chang-qing Gadd45b is a Novel Mediator of Neuronal Apoptosis in Ischemic Stroke |
title | Gadd45b is a Novel Mediator of Neuronal Apoptosis in Ischemic Stroke |
title_full | Gadd45b is a Novel Mediator of Neuronal Apoptosis in Ischemic Stroke |
title_fullStr | Gadd45b is a Novel Mediator of Neuronal Apoptosis in Ischemic Stroke |
title_full_unstemmed | Gadd45b is a Novel Mediator of Neuronal Apoptosis in Ischemic Stroke |
title_short | Gadd45b is a Novel Mediator of Neuronal Apoptosis in Ischemic Stroke |
title_sort | gadd45b is a novel mediator of neuronal apoptosis in ischemic stroke |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323375/ https://www.ncbi.nlm.nih.gov/pubmed/25678854 http://dx.doi.org/10.7150/ijbs.9813 |
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