Cargando…

CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2

NS2 from influenza A virus mediates Crm1-dependent vRNP nuclear export through interaction with Crm1. However, even though the nuclear export signal 1 (NES1) of NS2 does not play a requisite role in NS2–Crm1 interaction, there is no doubt that NES1 is crucial for vRNP nuclear export. While the mecha...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Yong, Liu, Xiaokun, Zhang, Anding, Zhou, Hongbo, Liu, Ziduo, Chen, Huanchun, Jin, Meilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Basel 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323543/
https://www.ncbi.nlm.nih.gov/pubmed/25213355
http://dx.doi.org/10.1007/s00018-014-1726-9
_version_ 1782356558393376768
author Hu, Yong
Liu, Xiaokun
Zhang, Anding
Zhou, Hongbo
Liu, Ziduo
Chen, Huanchun
Jin, Meilin
author_facet Hu, Yong
Liu, Xiaokun
Zhang, Anding
Zhou, Hongbo
Liu, Ziduo
Chen, Huanchun
Jin, Meilin
author_sort Hu, Yong
collection PubMed
description NS2 from influenza A virus mediates Crm1-dependent vRNP nuclear export through interaction with Crm1. However, even though the nuclear export signal 1 (NES1) of NS2 does not play a requisite role in NS2–Crm1 interaction, there is no doubt that NES1 is crucial for vRNP nuclear export. While the mechanism of the NES1 is still unclear, it is speculated that certain host partners might mediate the NES1 function through their interaction with NES1. In the present study, chromodomain-helicase-DNA-binding protein 3 (CHD3) was identified as a novel host nuclear protein for locating NS2 and Crm1 on dense chromatin for NS2 and Crm1-dependent vRNP nuclear export. CHD3 was confirmed to interact with NES1 in NS2, and a disruption to this interaction by mutation in NES1 significantly delayed viral vRNPs export and viral propagation. Further, the knockdown of CHD3 would affect the propagation of the wild-type virus but not the mutant with the weakened NS2–CHD3 interaction. Therefore, this study demonstrates that NES1 is required for maximal binding of NS2 to CHD3, and that the NS2–CHD3 interaction on the dense chromatin contributed to the NS2-mediated vRNP nuclear export. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00018-014-1726-9) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4323543
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Springer Basel
record_format MEDLINE/PubMed
spelling pubmed-43235432015-02-18 CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2 Hu, Yong Liu, Xiaokun Zhang, Anding Zhou, Hongbo Liu, Ziduo Chen, Huanchun Jin, Meilin Cell Mol Life Sci Research Article NS2 from influenza A virus mediates Crm1-dependent vRNP nuclear export through interaction with Crm1. However, even though the nuclear export signal 1 (NES1) of NS2 does not play a requisite role in NS2–Crm1 interaction, there is no doubt that NES1 is crucial for vRNP nuclear export. While the mechanism of the NES1 is still unclear, it is speculated that certain host partners might mediate the NES1 function through their interaction with NES1. In the present study, chromodomain-helicase-DNA-binding protein 3 (CHD3) was identified as a novel host nuclear protein for locating NS2 and Crm1 on dense chromatin for NS2 and Crm1-dependent vRNP nuclear export. CHD3 was confirmed to interact with NES1 in NS2, and a disruption to this interaction by mutation in NES1 significantly delayed viral vRNPs export and viral propagation. Further, the knockdown of CHD3 would affect the propagation of the wild-type virus but not the mutant with the weakened NS2–CHD3 interaction. Therefore, this study demonstrates that NES1 is required for maximal binding of NS2 to CHD3, and that the NS2–CHD3 interaction on the dense chromatin contributed to the NS2-mediated vRNP nuclear export. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00018-014-1726-9) contains supplementary material, which is available to authorized users. Springer Basel 2014-09-12 2015 /pmc/articles/PMC4323543/ /pubmed/25213355 http://dx.doi.org/10.1007/s00018-014-1726-9 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research Article
Hu, Yong
Liu, Xiaokun
Zhang, Anding
Zhou, Hongbo
Liu, Ziduo
Chen, Huanchun
Jin, Meilin
CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2
title CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2
title_full CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2
title_fullStr CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2
title_full_unstemmed CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2
title_short CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2
title_sort chd3 facilitates vrnp nuclear export by interacting with nes1 of influenza a virus ns2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4323543/
https://www.ncbi.nlm.nih.gov/pubmed/25213355
http://dx.doi.org/10.1007/s00018-014-1726-9
work_keys_str_mv AT huyong chd3facilitatesvrnpnuclearexportbyinteractingwithnes1ofinfluenzaavirusns2
AT liuxiaokun chd3facilitatesvrnpnuclearexportbyinteractingwithnes1ofinfluenzaavirusns2
AT zhanganding chd3facilitatesvrnpnuclearexportbyinteractingwithnes1ofinfluenzaavirusns2
AT zhouhongbo chd3facilitatesvrnpnuclearexportbyinteractingwithnes1ofinfluenzaavirusns2
AT liuziduo chd3facilitatesvrnpnuclearexportbyinteractingwithnes1ofinfluenzaavirusns2
AT chenhuanchun chd3facilitatesvrnpnuclearexportbyinteractingwithnes1ofinfluenzaavirusns2
AT jinmeilin chd3facilitatesvrnpnuclearexportbyinteractingwithnes1ofinfluenzaavirusns2