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Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells

BACKGROUND: Annona muricata (A. muricata) is widely distributed in Asia, Africa and South America. Different parts of this plant are used to treat several diseases in Cameroon. The aim of this study is to determine the in vitro anti-proliferative effects and apoptotic events of A. muricata extracts...

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Autores principales: Pieme, Constant Anatole, Kumar, Santosh Guru, Dongmo, Mireille Sylviane, Moukette, Bruno Moukette, Boyoum, Fabrice Fekam, Ngogang, Jeanne Yonkeu, Saxena, Ajit Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4324658/
https://www.ncbi.nlm.nih.gov/pubmed/25539720
http://dx.doi.org/10.1186/1472-6882-14-516
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author Pieme, Constant Anatole
Kumar, Santosh Guru
Dongmo, Mireille Sylviane
Moukette, Bruno Moukette
Boyoum, Fabrice Fekam
Ngogang, Jeanne Yonkeu
Saxena, Ajit Kumar
author_facet Pieme, Constant Anatole
Kumar, Santosh Guru
Dongmo, Mireille Sylviane
Moukette, Bruno Moukette
Boyoum, Fabrice Fekam
Ngogang, Jeanne Yonkeu
Saxena, Ajit Kumar
author_sort Pieme, Constant Anatole
collection PubMed
description BACKGROUND: Annona muricata (A. muricata) is widely distributed in Asia, Africa and South America. Different parts of this plant are used to treat several diseases in Cameroon. The aim of this study is to determine the in vitro anti-proliferative effects and apoptotic events of A. muricata extracts on HL-60 cells as well as to quantify its phenols content. METHODS: The cell viability was measured by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay while the changes in morphology of HL-60 cells, membrane mitochondrial potential (MMP) and the cell cycle were used for assessment apoptosis induction. RESULTS: The results show that the concentration of phenols, flavonoids and flavonols in the extracts varied depending on the part of the plant. All the extracts tested inhibited the proliferation of HL-60 cells in a concentration dependent manner with IC(50) varied from 6–49 μg/mL. The growth inhibition of the cells by extracts was associated with the disruption of MMP, reactive oxygen species (ROS) generation and the G0/G1 cell arrest. CONCLUSION: These findings suggest that the extracts from A. muricata have strong antiproliferation potential and can induce apoptosis through loss of MMP and G0/G1 phase cell arrest.
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spelling pubmed-43246582015-02-12 Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells Pieme, Constant Anatole Kumar, Santosh Guru Dongmo, Mireille Sylviane Moukette, Bruno Moukette Boyoum, Fabrice Fekam Ngogang, Jeanne Yonkeu Saxena, Ajit Kumar BMC Complement Altern Med Research Article BACKGROUND: Annona muricata (A. muricata) is widely distributed in Asia, Africa and South America. Different parts of this plant are used to treat several diseases in Cameroon. The aim of this study is to determine the in vitro anti-proliferative effects and apoptotic events of A. muricata extracts on HL-60 cells as well as to quantify its phenols content. METHODS: The cell viability was measured by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay while the changes in morphology of HL-60 cells, membrane mitochondrial potential (MMP) and the cell cycle were used for assessment apoptosis induction. RESULTS: The results show that the concentration of phenols, flavonoids and flavonols in the extracts varied depending on the part of the plant. All the extracts tested inhibited the proliferation of HL-60 cells in a concentration dependent manner with IC(50) varied from 6–49 μg/mL. The growth inhibition of the cells by extracts was associated with the disruption of MMP, reactive oxygen species (ROS) generation and the G0/G1 cell arrest. CONCLUSION: These findings suggest that the extracts from A. muricata have strong antiproliferation potential and can induce apoptosis through loss of MMP and G0/G1 phase cell arrest. BioMed Central 2014-12-24 /pmc/articles/PMC4324658/ /pubmed/25539720 http://dx.doi.org/10.1186/1472-6882-14-516 Text en © Pieme et al.; licensee BioMed Central. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Pieme, Constant Anatole
Kumar, Santosh Guru
Dongmo, Mireille Sylviane
Moukette, Bruno Moukette
Boyoum, Fabrice Fekam
Ngogang, Jeanne Yonkeu
Saxena, Ajit Kumar
Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells
title Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells
title_full Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells
title_fullStr Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells
title_full_unstemmed Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells
title_short Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells
title_sort antiproliferative activity and induction of apoptosis by annona muricata (annonaceae) extract on human cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4324658/
https://www.ncbi.nlm.nih.gov/pubmed/25539720
http://dx.doi.org/10.1186/1472-6882-14-516
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