Cargando…
Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats
Background. Severe burns result in hypercatabolic state and concomitant muscle atrophy that persists for several months, thereby limiting patient recovery. However, the effects of burns on the corresponding spinal dermatome remain unknown. This study aimed to investigate whether burns induce apoptos...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4324890/ https://www.ncbi.nlm.nih.gov/pubmed/25695065 http://dx.doi.org/10.1155/2015/372819 |
_version_ | 1782356743602307072 |
---|---|
author | Wu, Sheng-Hua Huang, Shu-Hung Cheng, Kuang-I Chai, Chee-Yin Yeh, Jwu-Lai Wu, Tai-Cheng Hsu, Yi-Chiang Kwan, Aij-Lie |
author_facet | Wu, Sheng-Hua Huang, Shu-Hung Cheng, Kuang-I Chai, Chee-Yin Yeh, Jwu-Lai Wu, Tai-Cheng Hsu, Yi-Chiang Kwan, Aij-Lie |
author_sort | Wu, Sheng-Hua |
collection | PubMed |
description | Background. Severe burns result in hypercatabolic state and concomitant muscle atrophy that persists for several months, thereby limiting patient recovery. However, the effects of burns on the corresponding spinal dermatome remain unknown. This study aimed to investigate whether burns induce apoptosis of spinal cord ventral horn motor neurons (VHMNs) and consequently cause skeletal muscle wasting. Methods. Third-degree hindpaw burn injury with 1% total body surface area (TBSA) rats were euthanized 4 and 8 weeks after burn injury. The apoptosis profiles in the ventral horns of the lumbar spinal cords, sciatic nerves, and gastrocnemius muscles were examined. The Schwann cells in the sciatic nerve were marked with S100. The gastrocnemius muscles were harvested to measure the denervation atrophy. Result. The VHMNs apoptosis in the spinal cord was observed after inducing third-degree burns in the hindpaw. The S100 and TUNEL double-positive cells in the sciatic nerve increased significantly after the burn injury. Gastrocnemius muscle apoptosis and denervation atrophy area increased significantly after the burn injury. Conclusion. Local hindpaw burn induces apoptosis in VHMNs and Schwann cells in sciatic nerve, which causes corresponding gastrocnemius muscle denervation atrophy. Our results provided an animal model to evaluate burn-induced muscle wasting, and elucidate the underlying mechanisms. |
format | Online Article Text |
id | pubmed-4324890 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43248902015-02-18 Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats Wu, Sheng-Hua Huang, Shu-Hung Cheng, Kuang-I Chai, Chee-Yin Yeh, Jwu-Lai Wu, Tai-Cheng Hsu, Yi-Chiang Kwan, Aij-Lie Biomed Res Int Research Article Background. Severe burns result in hypercatabolic state and concomitant muscle atrophy that persists for several months, thereby limiting patient recovery. However, the effects of burns on the corresponding spinal dermatome remain unknown. This study aimed to investigate whether burns induce apoptosis of spinal cord ventral horn motor neurons (VHMNs) and consequently cause skeletal muscle wasting. Methods. Third-degree hindpaw burn injury with 1% total body surface area (TBSA) rats were euthanized 4 and 8 weeks after burn injury. The apoptosis profiles in the ventral horns of the lumbar spinal cords, sciatic nerves, and gastrocnemius muscles were examined. The Schwann cells in the sciatic nerve were marked with S100. The gastrocnemius muscles were harvested to measure the denervation atrophy. Result. The VHMNs apoptosis in the spinal cord was observed after inducing third-degree burns in the hindpaw. The S100 and TUNEL double-positive cells in the sciatic nerve increased significantly after the burn injury. Gastrocnemius muscle apoptosis and denervation atrophy area increased significantly after the burn injury. Conclusion. Local hindpaw burn induces apoptosis in VHMNs and Schwann cells in sciatic nerve, which causes corresponding gastrocnemius muscle denervation atrophy. Our results provided an animal model to evaluate burn-induced muscle wasting, and elucidate the underlying mechanisms. Hindawi Publishing Corporation 2015 2015-01-28 /pmc/articles/PMC4324890/ /pubmed/25695065 http://dx.doi.org/10.1155/2015/372819 Text en Copyright © 2015 Sheng-Hua Wu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wu, Sheng-Hua Huang, Shu-Hung Cheng, Kuang-I Chai, Chee-Yin Yeh, Jwu-Lai Wu, Tai-Cheng Hsu, Yi-Chiang Kwan, Aij-Lie Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats |
title | Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats |
title_full | Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats |
title_fullStr | Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats |
title_full_unstemmed | Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats |
title_short | Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats |
title_sort | third-degree hindpaw burn injury induced apoptosis of lumbar spinal cord ventral horn motor neurons and sciatic nerve and muscle atrophy in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4324890/ https://www.ncbi.nlm.nih.gov/pubmed/25695065 http://dx.doi.org/10.1155/2015/372819 |
work_keys_str_mv | AT wushenghua thirddegreehindpawburninjuryinducedapoptosisoflumbarspinalcordventralhornmotorneuronsandsciaticnerveandmuscleatrophyinrats AT huangshuhung thirddegreehindpawburninjuryinducedapoptosisoflumbarspinalcordventralhornmotorneuronsandsciaticnerveandmuscleatrophyinrats AT chengkuangi thirddegreehindpawburninjuryinducedapoptosisoflumbarspinalcordventralhornmotorneuronsandsciaticnerveandmuscleatrophyinrats AT chaicheeyin thirddegreehindpawburninjuryinducedapoptosisoflumbarspinalcordventralhornmotorneuronsandsciaticnerveandmuscleatrophyinrats AT yehjwulai thirddegreehindpawburninjuryinducedapoptosisoflumbarspinalcordventralhornmotorneuronsandsciaticnerveandmuscleatrophyinrats AT wutaicheng thirddegreehindpawburninjuryinducedapoptosisoflumbarspinalcordventralhornmotorneuronsandsciaticnerveandmuscleatrophyinrats AT hsuyichiang thirddegreehindpawburninjuryinducedapoptosisoflumbarspinalcordventralhornmotorneuronsandsciaticnerveandmuscleatrophyinrats AT kwanaijlie thirddegreehindpawburninjuryinducedapoptosisoflumbarspinalcordventralhornmotorneuronsandsciaticnerveandmuscleatrophyinrats |